Microorganisms Isolated from the Slovak Traditional Cheese “Parenica”and their Identification with Mass Spectrometry

Authors

  • Miroslava Kačániová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Nitra 949 76, Tr. A. Hlinku 2, Slovakia
  • Margarita Terentjeva Latvia University of Life Sciences and Technologies, Faculty of Veterinary Medicine, Institute of Food and Environmental Hygiene, Jelgava, LV-3004, K. Helmaņa iela 8, Latvia
  • Simona Kunová Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Nitra 949 76, Tr. A. Hlinku 2, Slovakia
  • Ľudmila Nagyová Slovak University of Agriculture, Faculty of Economics and Management, Nitra 949 76, Tr. A. Hlinku 2, Slovakia
  • Elena Horská Slovak University of Agriculture, Faculty of Economics and Management, Nitra 949 76, Tr. A. Hlinku 2, Slovakia
  • Peter Haščík Slovak University of Agriculture, Faculty of Biotechnology and Food Sciences, Nitra 949 76, Tr. A. Hlinku 2, Slovakia
  • Maciej Kluz University of Rzeszow, Faculty of Biology and Agriculture, 35-601 Rzeszow, Zelwerowicza St. 4, Poland
  • Czeslaw Puchalski University of Rzeszow, Faculty of Biology and Agriculture, 35-601 Rzeszow, Zelwerowicza St. 4, Poland

Keywords:

cheese, microorganisms, identification, mass spectrometry

Abstract

Cheese is one of the most important products of dairy industry from the economic and nutritional points of view that demands the high processing technology and production hygiene. The aim of the study was to evaluate microbiological quality of traditional Slovak cheese “Parenica” produced from sheep milk. The coliforms, enterococci, lactic acid bacteria and microscopic filamentous fungi were detected in cheese samples. MALDI-TOF MS was applied for identification of isolates. In group of lactic acid bacteria three genera were identified: Lactococcus and Leuconostoc. Lactobacillus spp. was the most abundant genus, which was found in 25, Leuconostoc in 10 and Lactococcus in 7 samples. Lactobacillus was mostly represented with four species: Lactobacillus plantarum, L. acidophilus, L. ultunensis, L. casei. Another bacterial isolates included Gram-positive and Gram-negative Enterococcus faecium, Streptococcus, Raoutella ornithinolytica and Klebsiela oxytoca. Our study shows that MALDI-TOF MS could serve as an important method for identifying microorganisms for dairy industry needs.

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Published

2023-09-12