Activity of Origanum vulgare essential oil in liquid and vapor phase against pathogenic microorganisms

Lucia Galovičová

Abstract


The analysis was aimed to evaluate the antimicrobial activity of the essential oil Origanum vulgare against various gram-positive and gram-negative bacteria and yeast. To test its suitability in the vapor phase for the elimination of biofilm-forming bacteria on fruits and vegetables. To evaluate its potential for use in the storage of vegetables and fruits. The tested essential oil Origanum vulgare showed strong antimicrobial activity against various types of microorganisms. Due to the high antimicrobial activity against diverse group of microorganisms, it could also be a suitable option for the post-harvest treatment of crops in the future. In evaluating the inhibitory activity in the vapor phase, an effect on the biofilm-forming bacteria S. maltophilia and B. subtilis was demonstrated. Against S. maltophilia, the highest efficacy was found at a concentration of 500 µL/L by 91.27% inhibition of bacteria on potatoes. Against B. subtilis, the most effective concentration was 250 µL/L by 63.24 % of bacterial inhibition on carrots. Relatively stable growth inhibition was found in the apple sample at all concentrations tested. Due to the vaporous application, it would be possible to use lower concentrations of essential oils to achieve the antimicrobial activity. The impact on the sensory properties of food would be reduced.


Keywords


Origanum vulgare, biofilm, vapor phase, ex situ

Full Text:

PDF

References


Lukas, B., Schmiderer, C., Novak, J., Essential oil diversity of European Origanum vulgare L. (Lamiaceae), Phytochemistry, 2015, 119, 32-40. https://doi.org/10.1016/j.phytochem.2015.09.008

Burt, S., Essential oils: their antibacterial properties and potential applications in foods a review, International Journal of Food Microbiology, 2004, 94(3), 223-253. https://doi.org/10.1016/j.ijfoodmicro.2004.03.022

Debiagi, F., Kobayashi, R. K. T., Nakazato, G., Panagio, L. A., Mali, S., Biodegradable active packaging based on cassava bagasse, polyvinyl alcohol and essential oils, Industrial Crops and Products, 2014, 52, 664-670. https://doi.org/10.1016/j.indcrop.2013.11.032

Martínez, K., Ortiz, M., Albis, A., Castañeda, C. G. G., Valencia, M. E., Tovar, C. D. G., The Effect of Edible Chitosan Coatings Incorporated with Thymus capitatus Essential Oil on the Shelf-Life of Strawberry (Fragaria x ananassa) during Cold Storage, Biomolecules, 2018, 8(4), 155. https://doi.org/10.3390/biom8040155

Cui, H., Zhang, C., Li, C., Lin, L., Inhibition of Escherichia coli O157:H7 biofilm on vegetable surface by solid liposomes of clove oil, LWT - Food Science and Technology, 2020, 117, 108656. https://doi.org/10.1016/j.lwt.2019.108656

Oniga, I., Pușcaș, C., Silaghi-Dumitrescu, R., Olah, N. K., Sevastre, B., Marica, R., Marcus, I., Sevastre-Berghian, A. C., Benedec, D., Pop, C. E., Hanganu, D., Origanum vulgare ssp. vulgare: Chemical Composition and Biological Studies, Molecules, 2018, 23(8), 2077. https://doi.org/10.3390/molecules23082077

Benedec, D., Hanganu, D., Oniga, I., Tiperciuc, B., Olah, N. K., Raita, O., Bischin, C., Silaghi-Dumitrescu, R., Vlase, L., Assessment of rosmarinic acid content in six Lamiaceae species, Pakistan Journal of Pharmaceutical Sciences, 2015, 28(6), 2279-2303.

Teles, A. M., Rosa, T. D. S., Abreu-Silva, A. L., Calabrese, K. S., Almeida-Souza, F., Cinnamomum zeylanicum, Origanum vulgare, and Curcuma longa Essential Oils: Chemical Composition, Antimicrobial and Antileishmanial Activity extracts and their antioxidant and antimicrobial potential, Evidence-Based Complementary and Alternative Medicine, 2019, 2019, 12. https://doi.org/10.1155/2019/2421695

Enayatifard, R., Akbari, J., Babaei, A., Rostamkalaei, S. S., Hashemi, S. M. H., Habibi, E., Anti-Microbial Potential of Nano-Emulsion form of Essential Oil Obtained from Aerial Parts of Origanum Vulgare L. as Food Additive, Advanced Pharmaceutical Bulletin, 2021, 11(2), 327-334. https://doi.org/: 10.34172/apb.2021.028

Bhat, V., Sharma, S. M., Shetty, V., Shastry, C. S., Rao, C. V., Shenoy, S., Saha, S., Balaji, S., Characterization of Herbal Antifungal Agent, Origanum vulgare against Oral Candida spp. Isolated from Patients with Candida-Associated Denture Stomatitis: An In vitro Study, Contemporary Clinical Dentistry, 2018, 9, 3-10. https://doi.org/ 10.4103/ccd.ccd_537_17

Sarikurkcu, C., Zengin, G., Oskay, M., Uysal, S., Ceylan, R., Aktumsek, A., Composition, antioxidant, antimicrobial and enzyme inhibition activities of two Origanum vulgare subspecies (subsp. vulgare and subsp. hirtum) essential oils, 2015, 70, 178-184. https://doi.org/10.1016/j.indcrop.2015.03.030

Şahin, F., Güllüce, M., Daferera, D., Sökmen, A., Sökmen, M., Polissiou, M., Agar, G., Özer, H., Biological activities of the essential oils and methanol extract of Origanum vulgare ssp. vulgare in the Eastern Anatolia region of Turkey, Food Control, 2004, 15(7), 549-557. https://doi.org/10.1016/j.foodcont.2003.08.009

Nedorostova, L., Kloucek, P., Kokoska, L., Stolcova, M., Pulkrabek, J., Food Control, 2009, 20(2), 157-160. https://doi.org/10.1016/j.foodcont.2008.03.007

Doran, A. L., Morden, W. E., Dunn, K., Edwards‐Jones, V., Vapour–phase activities of essential oils against antibiotic sensitive and resistant bacteria including MRSA, Letters in Applied Microbiology, 2009, 48(4), 387-392. https://doi.org/10.1111/j.1472-765X.2009.02552.x

Laird, K., Phillips, C., Vapour phase: a potential future use for essential oils as antimicrobials?, Letters in Applied Microbiology, 2012, 54(3), 169-174. https://doi.org/10.1111/j.1472-765X.2011.03190.x


Refbacks

  • There are currently no refbacks.


Copyright (c) 2021 Scientific Papers Animal Science and Biotechnologies


Scientific Papers Animal Science and Biotechnologies publish open access articles under the terms of the Creative Commons Attribution 4.0 Licence (read full legal code).


LUCRARI STIINTIFICE ZOOTEHNIE SI BIOTEHNOLOGII (SCIENTIFIC PAPERS ANIMAL SCIENCE AND BIOTECHNOLOGIES)

ISSN print 1841 - 9364
ISSN online 2344 - 4576
ISSN-L  1841 - 9364
(former ISSN 1221-5287, E-ISSN 1841-9364)

PUBLISHER: AGROPRINT Timisoara, Romania
PAPER ACCESS: Full text articles available for free
FREQUENCY: Semiannual
PUBLICATION LANGUAGE: English
_________________________________________________________________

Banat´s University of Agricultural Sciences and Veterinary Medicine "King Michael I of Romania" from Timisoara
Bioengineering Faculty of Animal Resources
300645, Timişoara, Calea Aradului 119, Romania

E-mail: spasb [at] animalsci-tm.ro
Phone: +40-256-277160, Fax.: +40-256-277110