A Study of Phosphorus and Calcium Dynamics in an Integrated Rainbow Trout and Spinach (Nores variety) Aquaponic System with Different Crop Densities

Stefan Mihai Petrea, Victor Cristea, Lorena Dediu, Maria Contoman, Mirela Cretu, Alina Antache, Marian Coada, Alexandru Cristian Bandi


The goal of this study is to quantify both calcium and phosphorus budgets for an integrated rainbow trout – spinach (Nores variety) aquaponic system, where three crops densities were used (BH1 –59 crops/m2, BH2 – 48 crops/m2 and BH3 – 39 crops/m2 and a control variant). Fish were fed with two types of feed (41% and 50% protein), using 3 different feeding regimes. Total calcium and total phosphorus retention rates for each of the three tested spinach biomass densities were individually determined by water chemical and plant biochemical analysis. Also, the concentration of those two macroelements was determined from fish meat and fish faeces.  Significant differences (p<0.05) were recorded between fish faeces total phosphorus content and between total calcium and total phosphorus retention rates for each of the three variants of tested crops densities (significant higher at BH1 compared to BH3, p < 0.05). It is recommended that lower densities to be used for a better crop absorption of both calcium and phosphorus or a lower hydraulic flow regime and a better light intensity to be applied in case of the used integrated aquaponic system.


aquaponic system; calcium; phosphorus; rainbow trout; spinach; faeces.

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Masser, M.P., J. Rakocy, And T.M. Losordo; 1999; Recirculating Aquaculture Tank Production Systems: Management Of Recirculating Systems. S. Regional Aquaculture Center Publication No. 452:pp. 12.

Ghaly A.E., Kamal M., Mahmoud N.S.; 2005; Phytoremediation Of Aquaculture Wastewater For Water Recycling And Production Of Fish Feed; Environment International 31: pp. 1 – 13.

Rakocy J., Thomas M. Losordo, Michael P. Masser; 1992; Recirculating Aquaculture Tank Production Systems Integrating Fish And Plant Culture Srac Publication No. 454. pp:103

Nichols M.; 2008; Aquaponics: Where One Plus One Equals Three, Massey University, Palmerston North, New Zeeland, Maximum Yeld- Indoor Gradening, Uk, pp83.

Blidariu F., Grozea A.; 2011; Increasing The Economical Efficiency And Sustainability Of Indoor Fish Farming By Means Of Aquaponics– Review; Animal Science And Biotechnologies, 44 (2): pp. 1-8.

Pantanella E., Cardarelli M., Colla G., Rea E., Marcucci, A.; 2010 Aquaponics Vs Hydroponics: Production And Quality Of Lettuce Crop, Ishs Acta Horticulturae 927: Xxvii International Horticultural Congress On Science And Horticulture For People (Ihc2020) International Symposium On Greenhouse 2010 And Soilless Cultivation, pp: 1-8.

Blidariu F., Radulov I., Lalescu D., Drasovean A., Grozea A.; 2013; Evaluation Of Nitrate Level In Green Lettuce Conventional Grown Under Natural Conditions And Aquaponic System; Animal Science And Biotechnologies, 46 (1), pp. 244-250.

Dediu, L., Cristea, V., Xiaoshua, Z.; 2012; Waste Production And Valorization In An Integrated Aquaponic System With Bester And Lettuce. African Journal Of Biotechnology Vol. 11(9): 2349-2358.

Metaxa E, Deviller G, Pagand P, Alliaume C, Casellas C, Blancheton Jp; 2006; High Rate Algal Pond Treatment For Water Reuse In A Marine Fish Recirculation System: Water Purification And Fish Health. Aquaculture, 252; pp: 92-101.

Daniel P. Schachtman, Robert J. Reid, And S.M. Ayling; 1998; Phosphorus Uptake By Plants: From Soil To Cell; Plant Physiol. 116: 447–453;

Theodorou M., Plaxton W.; 1993; Metabolic Adaptations Of Plant Respiration To Nutritional Phosphate Deprivation. Plant Physiol 10; pp: 339–344

Nrc. 1993 National Research Council. Nutrients Requirements Of Fish. National Academy Press. p: 114

Lazzari R., Baldisserotto B.; 2008; Nitrogen And Phosphorus Waste In Fish Farming; B. Inst. Pesca, São Paulo, 34(4); pp: 591 – 600.

Jahan P.; Watanabe T., Satoh, S.H., Kiron, I.; 2002; Different Combinations Of Protein Ingredients In Carp Diets For Reducing Phosphorus Loading. Fisheries Science, Tokyo, 68; pp:595-602.

Ismail F., Anjum M.R., Mamon A.N., Kazi T.G.; 2011; Trace Metal Contents Of Vegetables And Fruits Of Hyderabad Retail Market; Pakistan Journal Of Nutrition 10 (4); pp: 365-372.

Petrea St.M., Cristea V., Dediu L., Contoman M., Lupoae P., Mocanu (Cretu) M. And Coada M.T.; Vegetable Production In An Integrated Aquaponic System With Rainbow Trout And Spinach; 2013; Bulletin Of University Of Agricultural Sciences And Veterinary Medicine Cluj-Napoca. Animal Science And Biotechnologies, P45-54.

Petrea St.M., Cristea V., Dediu L., Contoman M., Lupoae P., Mocanu (Cretu) M. And Coada M.T.; A Study Of Nitrogen Cycle In A Integrated Aquaponic System With Different Plant Densities; 2013; Bulletin Of University Of Agricultural Sciences And Veterinary Medicine Cluj-Napoca. Animal Science And Biotechnologies, pp:55-64.

Hayward R. S.; Noltie D. B.; Wang N.; 1997; Use Of Compensatory Growth To Double Hybrid Sunfish Growth Rates. Trans. Am. Fish. Soc. 126; pp: 316–322.

Endut A., Jusoh A., Ali N., Wan Nik W.B., Hassan A.; 2010; A Study On The Optimal Hydraulic Loading Rate And Plant Ratios In Recirculation Aquaponic System.Bioresource Technology 101; pp: 1511–1517.

Satoh S., Hernandez A., Tokoro T., Morishita Y., Kiron V., Watanabe T.; 2003; Comparison Of Phosphorus Retention Efficiency Between Rainbow Trout (Oncorhynchus Mykiss) Fed A Commercial Diet And A Low Fish Meal Based Diet; Aquaculture 224; pp: 271–282.

Rodehutscord M., Gregus, Z.; Pfeffer, E; 2000; Effect Of Phosphorus Intake On Faecal And Non-Faecal Phosphorus Excretion In Rainbow Trout (Oncorhynchus Mykiss) And The Consequences For Comparative Phosphorus Availability Studies. Aquaculture, Amsterdam, 188; pp:383-398.

Kim J.D.; Kaushik S.J.; Breque J.; 1998; Nitrogen And Phosphorus Utilization In Rainbow Trout (Oncorhynchus Mykiss) Fed Diets With Or Without Fish Meal. Aquatic Living Resources, Paris, 11(4); pp:261-264.

Green, J.A.; Brannon, E.L.; Hardy, R.; 2002; Effects Of Dietary Phosphorus And Lipid Levels On Utilization And Excretion Of Phosphorus And Nitrogen by Rainbow Trout (Oncorhynchus Mykiss). Aquaculture Nutrition, 8; pp:291-298

C. M. McCay, A. V. Tunison, Mary Crowell, And Henry Paul; 1936; The Calcium And Phosphorus Content Of The Body Of The Brook Trout In Relation To Age, Growth And Food; J. Biol. Chem. 114; pp:259-263.

Yoshikazu Nakamura, Juro Yamada ; 1980; Effects Of Dietary Calcium Levels, Ca/P Ratios, And Calcium Components On The Calcium Absorption Rate In Carp; Bull. Fac. Fish. Hokkaido Univ,31(4); pp: 277-282.

Kaya C., Higgs D., Kirnak H.; 2001; The effects of high salinity and supplementary phosphorus and potassium on physiology and nutrition development of spinach; bulg. j. plant physiol., 27(3–4); pp: 47–59.

Blidariu F., Drasovean A., Grozea A.; 2013; Evaluation Of Phosphorus Level In Green Lettuce Conventional Grown Under Natural Conditions And Aquaponic System; Bulletin Of University Of Agricultural Sciences And Veterinary Medicine Cluj-Napoca. Animal Science And Biotechnologies, pp;128-135.

Stephen J. Naylor, Richard D. Moccia,* And Gordon M. Durant; 1999; The Chemical Composition Of Settleable Solid Fish Waste (Manure) From Commercial Rainbow Trout Farms In Ontario, Canada; North American Journal Of Aquaculture 61:21-26.

Westerman, P W., J. M. Hinshaw, And J. C. Barker; 1993; Trout Manure Characterization And Nitrogen Mineralization Rate; J-K. Wang, Editor. Techniques For Modern Aquaculture. American Society Of Agricultural Engineers; pp35-43.

Olson, G. L.; 1992; The Use Of Trout Manure As A Fertilizer For Idaho Crops. In Blake. (1992).pp 198-205

Bergheim, A., Kristiansen R., Kelly L.; 1993; Treatment And Utilization Of Sludge From Landbased Farms For Salmon; In J-K. Wang, Editor. Techniques For Modern Aquaculture. American So Ciety Of Agricultural Engineers; pp486-495

Mudrak, V. A.; 1981; Guidelines For Economical Commercial Fish Hatchery Wastewater Treatment Systems. In L. J. Allen And E. C. Kinney, Editors. Proceedings Of The Bio-Engineering Symposium For Fish Culture. American Fisheries Society, Fish Culture Section, Bethesda, Maryland; pp: 174-182

Axler, R. E, C. Tikkanen, J. Henneck, J. Schuldt, McDonald M.E.; 1997; Characteristics Of Effluent And Sludge From Two Commercial Rainbow Trout Farms In Minnesota. Progressive Fish-Culturist 59; pp: 161

Moccia R., Bevan D, Reid G.; 2007; Composition Of Fecal Waste From Commercial Trout Farms In Ontario: Macro And Micro Nutrient Analyses And Recommendations For Recycling; Final Report Submitted To The:Ontario Sustainable Aquaculture Working Group Environment Canada And University Of Guelph; pp.7.

Bureau, D.P., Cho, C.Y.; 1999; Phosphorus Utilization By Rainbow Trout (Oncorhynchus Mykiss): Estimation Of Dissolved Phosphorus Waste Output. Aquaculture 179; pp: 127–140.


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