Physiological Indicators in the Welfare Assessment of Riding Horses
Keywords:
horse fitness, horse welfare, neutrophyle-lymphocyte ratio, riding horses, stress response, serum cortisolAbstract
The welfare assessment of horses includes the evaluation of their ability to cope with the challenges of their environment and of work given to them. The aim of this study was to evaluate the welfare of 12 clinically healthy horses from a riding school, using physiological indicators. Double determinations were made (in winter and in spring of 2011) by a set of two tests each time, before and after physical effort. The clinical parameters (heart and respiratory rate) were in the normal range at 20 minutes after effort, decreasing towards the resting values. Slightly higher values were recorded in the spring comparing with the winter, but the differences were not statistically significant (P>0.05). Even if were in the normal range, the results of the haematology tests and the blood sugar values showed clearly the response of the horses’ organisms to exercise. The serum cortisol concentrations presented an effort dependent elevation and higher values of the basic level were observed in the spring than in winter. The other stress-response indicator recorded, the neutrophyle-lymphocyte ratio, showed dynamics correlated with that of the cortisol level. The physiological indicators tested in this study proved their real value in the riding horses’ welfare assessment.
References
The FEI code of conduct for the welfare of the horse, latest version, 2011, Home page address: http://www.fei.org
EFSA/AHAW, Basic information for the development of the animal welfare risk assessment guidelines, NRCAWI, 06/01, 2006
Federation Equestre Internationale, Veterinary regulations, 11th edition, Printed in Switzerland, 2010.
The Merck Veterinary Manual, 9th edition, Merck&Co., Inc. Whitehouse station, N.J., U.S.A., 2005
Geor, R., Chilling out after exercise, Extension services, 2001, Article no. 897
Freeman, D.A., Cymbaluk, N. F., Schott, H.C., Clinical, biochemical and hygiene assessment of stabled horses provided continuous or intermittent access to drinking water, American Journal of Veterinary Research, 1999, 60, 1445-1450
Freeman, D.W., Topliff, D.R., Collier, M.A., Monitoring fittness of horses by heart rate, Oklahoma Cooperative Extension Fact Sheets, 2008
Popescu, S., Animal hygiene and environmental protection, vol I., Ed. Napoca Star, Cluj-Napoca, 2010
Zolovick, A.M., Upson, D.W., Eleftheriobu, E., Diurnal variation in plasma glucocorticosteroid levels in the horse (Equus caballus), Journal of Endocrinology, 1966, 35, 249-253
Fazio, E., Ferlazzo, A., Evaluation of stress during transport, Veterinary Research Communications, 2003, 27, 519-524
Stull, C.L., Spier, S.J., Aldridge, B. M., Blanchard M., Stott J. L., Immunological response to long-term transport stress in mature horses and effects of adaptogenic dietary supplementation as an immunomodulator, Equine Veterinary Journal, 2004, 36, 583-589
Fazio, E., Medica, P., Aronica, V., Grasso, L., Ferlazzo, A., Circulating β-endorphin, adrenocorticotrophic hormone and cortisol levels of stallions before and after short road transport: stress effect of different distances, Acta Veterinaria Scandinavica, 2008, 50-56
Alexander, S.L., Irvine, C.H., The effect of social stress on adrenal axis activity in horses: the importance of monitoring corticosteroid-binding globulin capacity, Journal of Endocrinology, 1998, 157, 425-432
Irvine, C.H.G., Alexander, S.L., Factors affecting the circadian rhythm in plasma cortisol concentrations in horses, Domestic Animal Endocrinology, 1994, 11, 227-238
Cooper, T.R., Trunkfield, H.R., Zanella, A.J., Booth, W.D., An enzyme-linked immunosorbent assay for cortisol in the saliva of man and domestic farm animals, Joutnal of Endocrinology, 1989, 123, R13-16
Marc, M., Parvizi, N., Ellendorff, F., Kallweit, E. Elsaesser, F., Plasma cortisol and ACTH concentrations in the warmblood horse in response to a standardized treadmill exercise test as physiological markers for evaluation of training, Journal of Animal Science, 2000, 78, 1936-1946
Irvine, C.H.G., Alexander, S.L., Factors affecting the circadian rhythm in plasma cortisol concentrations in horses, Domestic Animal Endocrinology, 1994, 11, 227-238
Lloyd, D.H., Littlewood, J.D., Craig, J.M., Thomsett, L.R., Blood cortisol. In: Practical equine dermatology, Blackwell Science, Iowa, U.S.A, 2003
Schwarzenberger, F., Möstl, E., Palme, R., Bamberg, E., Faecal steroid analysis for non-invasive monitoring of reproductive status in farm, wild and animals, Animal Reproduction Science, 42, 515-526 zoo animals, Animal Reproduction Science, 42, 515-526
Gill, J., Kompanowska-Jezierska, E., Jakubow, K., Kott, A., Szumska, D., Seasonal changes in the white blood cell system, lysozyme activity and cortisol level
in Arabian brood mares and their foals, Comparative Biochemical Physiology, 1985, 81A, 511-523
Thornton, J., Effect of the microclimate on horses during international air transportation in an enclosed container, Australian Veterinary Journal, 2000, 78, 472-477
Korhonen, P.A.S., Lilius, E.M., Hyyppä, S., Räsänen, L. A., Pösö, A. R., Production of reactive oxygen species in neutrophils after repeated bouts of exercise in standardbred trotters, Journal of veterinary medicine A Physiology, pathology, clinical medicine, 2000, 47, 565-73
Krumrych, W., Effect of Physical Exercise On Oxygen Metabolism Of Neutrophils In Horses, Bulletin of the Veterinary Institute Pulawy, 2009, 53, 97-10.