Comparative Analysis of Two DNA Extraction Methods Used in Genomic Research in Cattle
Keywords:
automatic extraction of DNA, deoxyribonucleic acid, genetic analysis, manual extraction of DNAAbstract
DNA (deoxyribonucleic acid) extraction is the first key step in the success of a genetic analysis. This research aims to validate the optimal method of DNA extraction from a number of 30 blood samples collected from Holstein cattle breed. Two methods of DNA extraction were tested, manual method (using Wizard Genomic DNA extraction kit) and automatic method (with Maxwell equipment). The results were interpreted statistically, finding that the average DNA concentration extracted by automatic method was 27.82 μg/µl compared to the average value of 18.01 μg/µl, obtained by manual method, the difference between the two values being quite high, 9.81 μg/µl. Following the application of the T-student test, with unequal variances, a value P = 1.41E-08 ˂ 0.05 resulted, which means that there are statistically significant differences regarding the concentration of the samples of DNA extracted by the two methods, the highest value of the concentration being obtained after the application of the automatic method. The accuracy of the results, the purity of the samples, the short analysis time and the lack of contamination of the samples are just some of the advantages of the automatic method of DNA extraction, which is recommended to be used in molecular genetics studies.
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