Histological Aspects Regarding the Antioxidant and Chelating Potential of Chlorella in Experimental Pb Contamination of Carassius gibelio Bloch species
Keywords:
chlorella, experimental lead intoxication, fish, histological lesionsAbstract
The purpose of this study was to highlight the histological aspect of some tissue of Prussian carp’s specimens, subjected to sub lethal Pb intoxication with and without chlorella dietary supplementation.
90 Prussian carps, with weighing between 10 and 12 g were divided according to the following treatments for 21 days: C (without treatment), E1 (75 ppm Pb into water), E2 (75 ppm Pb into water+2% lyophilized chlorella in feed).
Samples of skin, muscle and gonads were removed at the end of experimental period and analyzed in light microscopy and a specific QuickPHOTO Micro 2.2 software has been used for the histological study.
Mentioned epithelium suffered evident Pb-induced histomorphological alterations while the active principles of chlorella powder has attenuated the destructive action of lead to a greater or lesser extent, showing its chelating and antioxidant potential.
References
Greger, M., Metal Availability, Uptake, Transport and Accumulation in Plants. In: Heavy Metal Stress in
Plants, N. V. Prasad (ed.), © Springer-Verlag Berlin Heidelberg 2004, pp. 2
Adriano, D. C., Trace Elements in Terrestrial Environments-Biogeochemistry, Bioavailability and Risk of Metals (2nd Ed.), Springer-Valey, 2001, pp. 4-7
Iordache, V., Ecotoxicologia metalelor grele în lunca Dunării, Ed. Ars Docendi, 2009, pp. 20-21
Adams, M. L., McGrath, S. P., Zhao, F. J., Nicholson, F. A. , Sinclair, A. H., Lead and cadmium as contaminants in UK wheat and barley, HGCA Conference: Crop management into the Millenium, 2000
ILA (Internatioal Lead Association), Lead in Aquatic Environments, https://www.ila-lead.org/UserFiles/File/Aquatic_Environment_Report_v6_hi%20res.pdf
Mourente, G., Diaz-Salvago, E., Tocher, D. R., Bell, J. G, Effects of dietary polyunsaturated fatty acids/vitaminE (PUFA/tocopherol) ratio on antioxidant defense mechanisms of juvenile gilthead sea bream (Sparus aurata L., Osteichthyes, Sparidae), Fish Physiol. Biochem., 2000, 23, 337–351
Martinez-Alvarez, R. M., Morales, A. E., Sanz, A., Antioxidant Defenses in Fish: Biotic and Abiotic Factors, Fish Biology and Fisheries, 2005, 15, 75–88
Senug, H., Min-Won, B., Hui-Young, L., Dong-Jae, K., Yi-Rang, N., Kyoung, N., Song, P., Hyan, K., Byoung, H., Dong, R., Jae-Hak, P., Arsenite-induced apoptosis is prevented by antioxidants in zebra fish liver cell line, Toxicology in Vitro, 2007, 21(5), 870-877
Kim, Y. J., Kwon, S., Kim, M. K., Effect of Chlorella vulgaris intake on cadmium detoxification in rats fed cadmium, Nutr Res Pract., 2009, 3(2), 89–94.
Worku Sahu, O., Reduction of Heavy Metal and Hardness from Ground Water by Algae, Journal of Applied & Environmental Microbiology, 2014, 2(3), 86-89
Miranda, M. S., Sato, S., Mancini-Filho, J., Antioxidant activity of the microalga Chlorella vulgaris cultured on special conditions, Boll Chim Farm., 2001, 140(3), 165-8
Sivakumar Rajeshkumar Xiaoyu Li, Bioaccumulation of heavy metals in fish species from the Meiliang Bay, Taihu Lake, China, Toxicol Rep., 2018, 5, 288-295
Nicula, M., Dumitrescu, G., Păcală, N., Tulcan, C., Dragomirescu, M., Ştef, L., Dronca D., Gherbon A., Petculescu Ciochina, L., Garlic, cilantro and chlorella’s effect on gill histoarchitecture changes in Cd-intoxicated Prussian carp (Carassius gibelio), Romanian
Biotechnological Letters, 2016, 21(5), 11933
El-Serafy, S. S., Ibrahim, S. A., Mahmoud, S. A., Biochemical and histopathological studies on the muscles of the Nile tilapia (Oreochromis niloticus) in Egypt, Egyptian Journal of Aquatic Biology and Fisheries, 2005, 9(1), 81-96
Sitohy, M. Z., El-Masry, R. A., Siliem, T. A., Mohamed, N. A., Impact of some trace metals pollution in the River Nile water on muscles of Clarias gariepinus inhabiting El-Kanater El-Khyria and Helwan sites, Zaga Journal of Agricultual Research, 2006, 33(6),1207-1222
Saad, S. M. M., El-Deeb, A. E., Tayel, S. I., Al-Shehri, E., Ahmed, Nam, Effect of heavy metals pollution on histopathological alterations in muscles of Clarias gariepinus inhabiting the Rosetta branch, River Nile, Egypt, Ist International Conference of Biotechnology Applied Agriculture Benha University, Egypt, 2012, 79-88
Abbas, H, Ali, F., Study the effect of hexavalent chromium on some biochemical, cytological and histopathological aspects of Oreochromis spp. Fish, Pakistan Journal of Biological Sciences; 2007, 10, 3973-3982
Patnaik, B. B., Howrelia, J. H., Mathews, T., Selvanayagam, M., Histopathology of gill, liver, exposed to sublethal concentration of lead and cadmium, African Journal of Biotechnology, 2011, 10(57), 12218-12223
Fatma, A. S. M., Histopathological studies on Tilapia zillii and Solea vulgaris from Lake Qarun, Egypt, World Journal of Fish and Marine Sciences, 2009, 1(1), 29-39
Doaa, M., Hanan, H., Histological Changes in Selected Organs of Oreochromis niloticus Exposed to Doses of Lead Acetate, J. Life Sci. Biomed., 2013, 3(3), 256-263
Salim, F., Histopathological Effect of heavy metal on different organs of fresh water fish tissues from Garmat Ali River adjacent to Al- Najebyia Power Station, Kufa Journal For Veterinary Medical Sciences, 2015, 6 (1), 141-153
