Identification of Concentration Dependent in vitro effect of Bisphenol F on H295R Cell Viability, Membrane Integrity and Lysosomal Function
Keywords:
endocrine disruptors, BPF, H295R, viability, membrane integrity, lysosomal functionAbstract
In recent years, the use of Bisphenol A (BPA) has been regulated in many countries because of its potential adverse effects on human health. As a result of the restriction, structural analogues such as bisphenol F (BPF) have already been used for industrial applications as alternatives to BPA. Although much information on the endocrine activity of BPA is available, a proper human hazard assessment of analogues that are believed to have a less harmful toxicity profile is lacking. The aim of our in vitro study was to assess the potential effect of BPF on H295R cell viability, membrane integrity and lysosomal function. Adrenocortical carcinoma cells were cultivated during 24 h in the presence of BPF (0.1, 0.5, 1, 10, 25, 50, 75, 100, 300, 500 μM). Metabolic activity decreased with increasing dose of BPF - from 10 μM (84.33 ± 4.31%). A significant increase in metabolic activity after 24 hours of exposure was observed after cultivation with 0.1 μM BPF (111.50 ± 3.89%) and a slight was observed after cultivation with 1 μM BPF (101,70 ± 1.61%). Exposure doses of BPF caused a slight increase in esterase activity at the lowest concentrations and a significant decrease at higher concentrations. We observed a slight increase in lysosomal function after cultivation with 0.1 and 1 μM, higher exposure doses (25 - 500 μM) caused decrease in lysosomal function. The obtained results confirmed that BPF at higher concentrations caused cytotoxicity. A substitution of BPA by BPF should be thus considered with caution.
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