Effect of Chickpea and Probiotics on Broiler Chicks’ Performance and Gut Microflora Populations
Keywords:
broiler, chickpea, gut microflora, probioticAbstract
This study aimed to investigate, for a period of 42 d, the effect of different levels (0, 10 and 20%) of raw chickpeas (CKP; Cicer arietinum L., cv. Burnas) seeds, as replacement of SBM, with and without probiotic addition on growth performance (GP). The impact of the treatments on the cecal microflora population, and pH at d 26 was also evaluated. A total of 720, unsexed 1-d-old Cobb 500 chicks were divided into 6 groups with 4 replicate pens (30 birds/replicate pen). Data were analyzed as a 3 x 2 factorial arrangement [3 levels of CKP with and without probiotic (3x108 cfu /kg diet of Lactobacillus plantarum ATCC 8014)]. The results showed that CKP up to 20% without probiotic in an optimized diet on digestible amino acid contents maintained broiler GP (2712.7-2696.2 g vs. 2718.5 g in SBM diet; P>0.05). The digestive organ sizes (i.e. gizzard, heart, liver, pancreas, small intestine, caecum) and pH of the cecal digesta were not affected by treatments. Probiotic addition was beneficially in modulating gut microflora composition. In particular, the cecal Lactobacillus (log10 cfu/g of wet digesta) and Enterococcus increased (P=0.04; P=0.52, respectively), whereas the coliforms decreased (P>0.05), compared with those without probiotic. It is concluded that probiotic addition in CKP diets had a beneficial effect on GP responses and cecal microflora populations.
References
Peng, Q., Zeng, X. F., Zhu, J. L., Wang, S., Liu, X. T., Hou, C. L., and Qiao, S. Y., Effects of dietary Lactobacillus plantarum B1 on growth performance, intestinal microbiota, and short chain fatty acid profiles in broiler chickens, Poultry Science, 2016, 95(4), 893-900.
Incharoen, T., Charoensook, R., Onoda, S., Tatrakoon, W., Numthuam, S., and Pechkong, T., The effects of heat-killed Lactobacillus plantarum L-137 supplementation on growth performance, intestinal morphology, and immune-related gene expression in broiler chickens, Anim. Feed Sci. Technol., 2019, https://doi.org/10.1016/j.anifeedsci.2019.114272
Saarela, M., Hallamaa, K., Mattila-Sandholm, T. and Matto, J., The effect of lactose derivatives lactulose, lactitol and lactobionic acid on the functional and technological properties of potentially probiotic Lactobacillus strains, Int. Dairy J., 2003, 13, 291-302.
Pender, C. M., Kim, S., Potter, T. D., Ritzi, M. M., Young, M., and Dalloul, R. A., Effects of in ovo supplementation of probiotics on performance and immunocompetence of broiler chicks to an Eimeria challenge, Beneficial Microbes, 2016, 7(5), 699-705.
FAO, 2016. Probiotics in Animal Nutrition-Production, Impact and Regulation. Home page address http://www.fao.org/3/a-i5933e.pdf
De Vries, M. C., Vaughan, E. E., Kleerebezem, M., and de Vos W. M., Lactobacillus plantarum-survival, functional and potential probiotic properties in the human intestinal tract, Int. Dairy J., 2006, 16(9), 1018-1028.
Wu, X. Z., Wen, Z. G., and Hua J. L., Effects of dietary inclusion of Lactobacillus and inulin on growth performance, gut microbiota, nutrient utilization, and immune parameters in broilers, Poultry Science, 2019, 98(10), 4656-4663.
Farrell, D. J., Perez-Maldonado, R. A., and Mannion, P. F., Optimum inclusion of field peas, faba beans, chick peas and sweet lupins in poultry diets. II. Broiler experiments, British Poultry Science, 1999, 40(5), 674-680.
Viveros, A., Brenes, A., Elices, R., Arija, I., and Canales, R., Nutritional value of raw and autoclaved kabuli and desi chickpeas (Cicer arietinum L.) for growing chickens. British Poultry Science, 2001, 42(2), 242-251.
Brenes, A., Viveros, A., Centeno, C., Arija, I., and Marzo, F., Nutritional value of raw and extruded chickpeas (Cicer arietinum L.) for growing chickens, Spanish J. Agric. Res., 2008, 6(4), 537-545.
Christodoulou, V., Bampidis, V. A., Hucko, B., Iliadis, C., and Mudrik, Z., Nutritional value of chickpeas in rations of broiler chickens, Arch. Geflügelk., 2006, 70(3), 112-118.
Katogianni, I., Zoiopoulos, P. E., Adamidis, C., and Fegeros, K., Substituting chickpeas for soybeans in diets for broilers fattened according to the European Community organic regime, Arch. Geflügelk., 2008, 72 (4), 152-156.
Bampidis, V. A., and Christodoulou, V., Chickpeas (Cicer arietinum L.) in animal nutrition: A review, Anim. Feed Sci. Technol., 2011, 168 (1-2):1-20.
Ciurescu, G., Vasilachi, A., and Grosu, H., Efficacy of microbial phytase on growth performance, carcass traits, bone mineralization and blood biochemistry parameters in broiler turkeys fed raw chickpeas (Cicer arietinum L., cv. Burnas) diets, J. Appl. Poult. Res., 2020, 29(1), 171-184.
Sorescu, I., Dumitru, M., and Ciurescu, G., Lactobacillus spp. and Enterococcus faecium strains isolation, identification, preservation and quantitative determinations from turkey gut content, Rom. Biotechnol. Lett., 2019, 24(1), 41-49.
Dumitru, M., Sorescu, I., Hăbeanu, M., Tabuc, C., Idriceanu, L and Jurcoane, S., Preliminary characterisation of Bacillus subtilis strain use as a dietary probiotic bio-additive in weaning piglet, Food and Feed Res., 2018, 45(2), 203-211.
Mountzouris, K. C., Tsirtsikos, P., Palamidi, I., Arvaniti, A., Mohnl, M., Schatzmayr, G., and Fegeros, K., Effects of probiotic inclusion levels in broiler nutrition on growth performance, nutrient digestibility, plasma immunoglobulins, and cecal microflora composition, Poultry Science, 2010, 89, 58-67.
SPSS, 2011. Statistics for Windows, Version 20.0. IBM Inc., Chicago, IL, USA.
Ciurescu, G., Vasilachi, A., Hăbeanu, M., and Dragomir, C., Effects of dietary lentil seeds inclusion on performance, carcass characteristics and cecal pH of broiler chickens, Indian J. Anim. Sci., 2017, 87(9), 1130-1134.
Gareau, M. G., Sherman, P. M., and Walker, W. A., Probiotics and the gut microbiota in intestinal health and disease, Nat. Rev. Gastroenterol. Hepatol., 2010, 7(9), 503-514.
Reis, M. P., Fassani, E. J., Júnior, A. A. P. G., Rodrigues, P. B., Bertechini, A. G., Barrett, N., and Schmidt, C. J., Effect of Bacillus subtilis (DSM 17299) on performance, digestibility, intestine morphology, and pH in broiler chickens. J. Appl. Poult. Res., 2017, 26(4), 573-583.
Jadhav, K., Sharma, K. S., Katoch, S., Sharma, V. K., and Mane, B. G., Probiotics in broiler poultry feeds: A Review, J. Anim. Nutr. Physiol., 2015, 1, 4-16.
