Effects of Dietary n-3 Rich PUFA Mixture on Performance, Plasma Metabolites and Muscle Fatty Acids Profile in Growing Pigs
Keywords:
meat fatty acids, performance, plasma parameters, n-3 PUFA mixAbstract
The study was conducted to assess the effect of feeding an n-3 PUFA-enriched diet on growth performance, plasma metabolites, and muscle fatty acids composition in growing pigs. Forty growing pigs (13.1±1.8 kg body weight) were randomly divided to receive a control [C based on corn-triticale-soybean meal (SBM)] or n-3 PUFA mix diet [extruded linseed EL: walnut meal WM, where the 80 g/kg dietary mixture (50:30 wt/wt) partially replace SBM] for 30 days. At the end of the trial, blood and muscle samples were collected for plasma profiles and fatty acids analyses. Feeding pigs with n-3 PUFA-enriched diet slightly improved body weight and average daily gain (P>0.05) with similar feed intake, and tend to improve feed efficiency (P=0.058). There was no effect on plasma protein profile, but the plasma urea nitrogen was decreased (P=0.042) indicating a higher utilization of dietary nitrogen. Plasma lipid profile was affected as a response to the dietary mixture by reducing the total cholesterol concentration (P=0.04) vs C diet. The Longissimus Dorsi muscle of pigs fed dietary mixture had higher contents in alpha-linolenic acid (P=0.030), docosahexaenoic (P=0.050), total PUFA (P=0.0001) and n-3 PUFA (P=0.019), and a lower n-6: n-3 ratio (P=0.002) vs pigs fed C diet. In conclusion, the dietary mixture had a positive effect on growing pigs’ performance and health status, improving the plasma lipo-protein response and enhanced the fatty acid profile of the meat.
References
Hăbeanu, M., Lefter, N., Gheorghe, A., Tabuc, C., Dumitru, M., Ciurescu, G., Palade, M., Effects of dietary peas mixed with linseed (3:1) on the growth performance, enteritis and certain serum parameter in weaned piglets, Food and Feed Research, 2017, 44(2), 173–180.
Taranu, I., Habeanu, M., Palade, L. M., Marin, D., Beneficial effect of dietary bioactive compounds from
residual nuts, a by-product of pastry on antioxidant defense in pigs after weaning, Scientific Papers: Animal Science and Biotechnologies, 2018, 51(1), 50–54.
Quiniou, N., Goues, T., Vautier, A., Nassy, G., Chesneau, G., Weill, P., Etienne, M., Mourot, J., Consequence of extruded linseed incorporation in sows and/or pigs' diets on performance. Conference at the 61st Annual Meeting of the European Association for Animal Production, Heraklion, Crete Island, Greece, 2010, pp. 5.
Beckova, B., Vaclavkova, E., The effect of linseed diet on carcass value traits and fatty acid composition in muscle and fat tissue of fattening pigs, Czech Journal of Animal Science, 2010, 55, 313–320.
Jasinska, K., Kurek, M.A., The effect of oil plants supplementation in pig diet on quality and nutritive value of pork meat, Animal Science Papers and Reports, 2017, 35, 137–146.
Barceló-Coblijn, G., Murphy, E. J., Othman, R., Moghadasian, M. H., Kashour, T., Friel, J. K., Flaxseed oil and fish-oil capsule consumption alters human red blood cell n-3 fatty acid composition: a multiple-dosing trial comparing 2 sources of n-3 fatty acid, The American Journal of Clinical Nutrition, 2008, 88(3), 801-809.
Goyal, A., Sharma, V., Upadhyay, N., Gill, S., Sihag, M., Flax and flaxseed oil: an ancient medicine & modern functional food, 2014, The Journal of Food Science and Technology, 51(9), 1633–1653.
Taranu, I., Gras, M., Pistol, G. C., Motiu, M., Marin,
D. E, Omega-3 PUFA rich camelina oil by-products improve the systemic metabolism and spleen cell functions in fattening pigs, PLoSOne, 2014, 9(10), e110186.
Wood, J. D., Richardson, R. I., Nute, G. R., Fisher,
V., Campo, M. M., Kasapidou, E., Effects of fatty acids on meat quality: a review, Meat Science, 2003, 66, 21–32.
Urrutia, O., Mendizabal, J. A., Insausti, K., Soret, B., Purroy, A., Arana, A., Effects of addition of linseed and marine algae to the diet on adipose tissue development, fatty acid profile, lipogenic gene expression, and meat quality in lambs, PLoSOne, 2016, 11(6), e0156765.
Hăbeanu, M., Lefter, N. L., Gheorghe, A., Untea, A., Ropotă, M., Grigore, D. M., Vărzaru, I., Toma, S. M., Evaluation of performance, nitrogen metabolism and tissue composition in barrows fed an n-3 PUFA- rich diet, Animals, 2019, 9, 234.
Jahanban-Esfahlan, A., Ostadrahimi, A., Tabibiazar M., Amarowicz, R., A comparative review on the extraction, antioxidant content and antioxidant potential of different parts of walnut (Juglans regia L.) Fruit and tree, Molecules, 2019, 24(11), 2133.
Fernández-Agulló, A., Pereira, E., Freire, M. S., Valentão, P., Andrade, P. B., González-Álvarez, J., Pereira J. A., Influence of solvent on the antioxidant and antimicrobial properties of walnut (Juglans regia L.) green husk extracts, Industrial Crops and Products, 42, 126-132.
Habeanu, M., Voicu, D., Lefter, N., Gheorghe, A., Ropota, M., Vlad, C., Ghita, E., Effects of the full fat walnuts waste on the fatty acids composition of the hepatic and cardiac tissue in Topigs piglets, 66th Annual Meeting of the European Association for Animal Production, Warsaw, Poland, 2015, pp. 267.
Gheorghe, A., Hăbeanu, M., Lefter, N. L., Grigore, D. M., Effect of dietary extruded linseed and walnut meal mixture (8:1) on performance and plasma protein profile in weaned piglets, Bulletin UASVM Animal Science and Biotechnologies, 2018, 75(2), 121–126.
Official Journal of the European Union, Directive 2010/63/EU of the European Parliament and of the Council on the Protection of Animals Used for Scientific Purposes, 2010, Series L 276, 33-79.
Nutrient Requirements of Swine, Revised 10th ed., National Academy Press, Washington, DC, 1998.
Official Journal of the European Union L 54, Commission Regulation (EC) No. 152/2009 laying down the methods of sampling and analysis for the official control of feed, 2009, Series L 155:1–66.
Hăbeanu, M., Lefter, N., Gheorghe, A., Al. Nagy, Marin, D., Ropotă, M., Effects of dietary flaxseed oil on the muscle fatty acid composition in Mangalitsa pigs in an extensive rearing system, South African Journal of Animal Science, 2014, 44(3), 240–244.
Tarricone, S., Colonna, M. A., Giannico, F., Ragni, M., Lestingi, A., Facciolongo, A. M., Effect of an extruded linseed diet on meat quality traits in Nero Lucano pigs, South African Journal of Animal Science 2019, 49 (6), 1093-1103.
Merck&Co., The Merck Veterinary Manual, 10th ed., Merck Co., Inc., Kenilworth, NJ, USA, 2010.
Perri, A. M., O’Sullivan, T. L., Harding, J. C. S, Wood, R. D., Friendship, R. M., Haematology and biochemistry reference intervals for Ontario commercial nursing pigs close to the time of weaning. Canadian Veterinary Journal, 2017, 58(4), 371–376.
Washington, I. M., Van Hoosier, G., The laboratory rabbit, guinea pig, hamster, and other rodents, Clinical Biochemistry and Hematology, 2012, doi: 10.1016/B978-0-12-380920-9.00003-1.
Kozera, W. J., Karpiesiuk, K. D., Bugnacka, J. Falkowski, Milewska, W, Production performance of pigs reared in different systems and fed increased energy content diets with or without green alfalfa, South African Journal of Animal Science, 2016, 46 (1), 70-76.
Gheorghe, A., Hăbeanu, M., Lefter, N. A., Dumitru, M., Grigore, D. M., Aspects of plasma biochemistry and intestinal health of weaned piglets fed dietary extruded linseed and walnut meal mixture, Food and Feed Research, 2019, 46(1), 147-153.
Hellwing, A., Louise, F., Tauson, A.H., Skrede, A., Blood parameters in growing pigs fed increasing levels of bacterial protein meal. Brief communication, Acta Veterinaria Scandinavica, 2007, 49(33), 1-4.
Shen, Y. B., Voilque, G., Kim, J. D., Odel, J., Kim, S. W., Effects of increasing tryptophan intake on growth and physiological changes in nursery pigs, Journal of Animal Science, 2012, 90, 2264-2275.
Tietz, N. W., Fundamentals of clinical chemistry. 4th ed. Philadelphia: W.B. Saunders Company, 1996.
Nevrkla, P., Vaclavkova, E., The effect of diet supplementation with linseed scrap on the meat quality and fatty acid profile of the meat and backfat in fattening gilts, Veterinarni Medicina, 2019, 64, 467–475.
Corino, C., Musella, M. Mourot, J., Influence of extruded linseed on growth, carcass composition and meat quality of slaughtered pigs at one hundred and one hundred sixty kilograms of liveweight, Journal of Animal Science, 2008, 86, 1850–1860.
Juárez, M., Dugan, M. E. R., Aldai, N., Aalhus, J. L., Patience, J. F., Zijlstra, R. T., Beaulieu, A. D., Increasing omega-3 levels through dietary co-extruded flaxseed supplementation negatively affects pork palatability, Food Chemistry, 2011, 126, 1716–1723.
De Tonnac, A., Karim-Luisset, S., Mourot, J., Effect of different dietary linseed sources on fatty acid composition in pig tissues, Livestock Science, 2017, 203, 124–131.
