In Vitro Determination of Wheat Dry Matter Solubility and Protein Digestibility
Keywords:
dry matter solubility, in vitro digestion, protein digestibility, wheatAbstract
The objectives of the present study were to determine the in vitro dry matter (DM) solubility and protein digestibility (PD) of wheat grains. Two experiments were conducted. In experiment 1, samples were incubated for different time periods with pepsin, simulating gastric digestion, and in experiment 2, samples were digested following an in vitro two-step procedure, simulating gastric and small intestine digestion. DM solubility in gastric digestion showed an increase with the incubation time. DM solubility of wheat ranged in experiment 1 from 0.1532 g/g at 30 minutes digestion time, to 0.1714 g/g at 120 minutes digestion time. Samples showed higher DM solubility and PD after small intestine incubation than after gastric incubation. DM solubility increased with 16.67% and PD increased with 24.87% when gastric digestion was followed by 240 minutes intestinal digestion.
References
Khan, M. A, Mahr-Un-Nisa, and M. Sarwar, M., Techniques Measuring Digestibility for the Nutritional Evaluation of Feeds, International Journal of Agriculture & Biology, 2003, 5(1), 91–94.
Martens, D. R. 2005. Rate and extent of digestion. In: Dijkstra, J., Forbes, J. M., and France, J. (eds.) Quantitative aspects of ruminant digestion. CABI International, Wallingford, UK. 2nd edition, pp. 13–47.
Boisen, S., Eggum, B. O., Critical evaluation of in vitro methods for estimating digestibility in simple-stomach animals, Nutrition Research Reviews, 1991, 4, 141-162.
Löwgren, W., Graham, H., and Aman, P., An in vitro method for studying digestion in the pig. Simulating digestion in the different compartments of the intestine, British Journal of Nutrition, 1989, 61, 673–687.
Boisen, S., Fernández, J.A., Prediction of the total tract digestibility of energy in feedstuffs and pig diets by in vitro analyses, Animal Feed Science and Technology, 1997, 68, 277–286.
Dierick, N., Vervaeke, I., Decuypere, J., and Hendrickx, H., Protein digestion in pigs measured in vivo and in vitro. In: Digestive Physiology in the Pig, A. Just, H. Jerrgensen and J. A. Fernandez eds., National Institute of Animal Science, Copenhagen, 1985, pp. 329-332.
Sauer, W. C., Jorgensen, H., Berzins, R., A modified nylon bag technique for determining apparent digestibilities of protein in feedstuffs for pigs, Canadian Journal of Animal Science, 1983, 63, 233-237.
Sauer, W. C., den Hartog, L. A., Huisman, J., van Leeuwen, P., and de Lange, C. F. M., The evaluation of the mobile nylon bag technique for determining the apparent protein digestibility in a wide variety of feedstuffs for pigs, Journal of Animal Science, 1989, 67, 432-440.
Pujol, S., Torrallardona, D., Evaluation of in vitro methods to estimate the in vivo nutrient digestibility of barley in pigs, Livestock Science, 2007, 109, 186–188.
Noblet, J., Jaguelin-Peyraud, Y., Prediction of digestibility of organic matter and energy in the growing pig from an in vitro model, Animal Feed Science and Technology, 2007, 134, 211–222.
Wilfart, A., Jaguelin-Peyraud, Y., Simmins, H., Noblet, J., van Milgen, J., and Montagne, L., Kinetics of enzymatic digestion of feeds as estimated by a stepwise in vitro method, Animal Feed Science and Technology, 2008, 141, 171–183.
AOAC, Association of Official Analytical Chemists. Official Methods of Analysis, K Helrick, ed. 13th edition. Arlington, 1990, pp. 1018.
