Preliminary Researches of Sperm Cryopreservation on Poliodon (Polyodon spathula) for Optimization of Spawning Biotechnology
Keywords:
cryopreservation, fertilization, motility, poliodon, spawnAbstract
The paper presents the first experiments of sperm cryopreservation on poliodon (Polyodon spathula), recently acclimated sturgeon in Romania. The accomplished research has been to determine the effect of using methanol (MeOH) and dimethyl sulfoxide (DMSO) as cryoprotectants in combination with two extenders (modified Tsvetkova’s extender: mT and modified Hanks’ balanced salt solution: mHBSS) on the spermatozoa motility and fertilization and hatching percentage of eggs by use of thawed sperm.
The best post thawed motility (78±5%) was recorded using modified Tsvetkova’s extender: mT with 1% MeOH, and the best fertilization and hatching percentage of eggs were achieved by using mT extender in combination with 5% MeOH. The results confirm data from literature (Detlaf et al. 1993, Mims et al. 2000, Akos Horvath et al. 2006) reporting that MeOH is a proper cryoprotectant for freezing poliodon sperm.
References
Dettlaff, T.A., Ginsburg A.S., and Schmalhausen O.I., Sturgeon fishes, developmental biology and aquaculture. Springer-Verlag, Berlin, Germany, 1993.
Brown, G.G. and Mims S.D., Cryopreservation of paddlefish Polyodon spathula milt. Journal of the World Aquaculture Society, 1999, 30, 245–249.
Horvath, A., W.R. Wayman, B. Urbanyi, K.M. Ware, J., Dean, C., and Tiersch, T.R., The relationship of cryoprotectants methanol and dimethyl sulfoxide and hyperosmotic extenders on sperm cryopreservation of two North-American sturgeon species. Aquaculture, 2005, 247, 243–251.
Mims, S.D., Tsvetkova L. I., Brown G.G., and Gomelsky B.I., Cryopreservation of sperm of sturgeon and paddlefish. Pages 123–129 in T. R. Tiersch and P. M. Mazik, editors. Cryopreservation in aquatic species. World Aquaculture Society, Baton Rouge, Louisiana, USA, 2000,
Stech, L., Linhart O., Shelton W.L., and Mims S.M., Minimally invasive surgical removal of ovulated eggs from paddlefish. Aquaculture International, 1999, 7, 129–133.
