Use of Biological Additives with Grass Containing Medium and High Levels of WSC for Effective Conservation and Aerobic Stability
Keywords:
aerobic stability, grass silage, Lactobacillus buchneri, Propionibacterium acidipropionici WSCAbstract
The objective of the trials was to determine the effect of two silage inoculant strains Lactobacillus buchneri and Propionibacteria acidipropionici on grass containing medium and high levels of WSC for fermentation characteristics and aerobic stability of silages. The basic raw materials originated from second growth cut grasses from 2 different plots of the farm: 1st.grass components were a mixture of grass- and leguminous species, contained medium 1.5-3.0% WSC/FM. The 2nd grass components was mainly grass species; with high>3% WSC/FM content.
We stored the filled micro (4.2 litre) containers on ambient temperature. It was proven that both inoculant strains significantly decreased lactic acid content (P<0.01) and increased acetic acid content (P<0.001) of silages and significantly increased the aerobic stability as well. The best aerobic stability>240 hours was the Lactobacillus buchneri treated silages originated from medium WSC/FM grass. The main advantage of treatment of L. buchneri on grass compared to P. acidipropionici is the longer aerobic stability of silage (P<0.001).
There was no significant differences among the microbiological profile neither of treated nor of control silages.
References
Archundia, M.E.U., Bolsen, K.K., Aerobic deterioration of silage: processes and prevention, 2007, http://en.engormix.com
Ashbell, G. and Weinberg, Z.G., Top silage losses in horizontal silos, Can. Agric. Eng., 1992, 34, 171-175
Kung, Jr. L., Muck, R.E., Animal response to silage additives. In: Proc. Natl. Silage Prod. Conf., Hershey, PA. NRAES-99. Northeast Reg. Agric. Ext. Serv., Ithaca, NY., 1997, pp. 200-210.
Honig, H., Pahlow, G., Thaysen, J., Aerobic instability – Effects and possibilities for its prevention. The XII International Silage, Conference. Uppsala, Sweden, 1999, pp. 288-289.
Ohyama, Y., Masaki, S., Hara, S., Factors influencing aerobic deterioration of silages and changes in chemical composition after opening silos, J. Sci. Food Agric., 1975, 26, 1137-1147.
Spoelstra, S.F., Courtin, M.G., Van Beers, J.A.C., Acetic acid bacteria can initiate aerobic deterioration of whole crop maize silage, J. Agric. Sci., Camb., 1988, 111, 127-132
Ruser, B., Kleiman, J., The effect of acetic acid on the aerobic stability of silages and on intake Proc. XIVth International Silage Conference, Belfast Northern Ireland, 2005, 231
Oude Elferink, S.J.W.H., Krooneman, J.C., Gottschal, J. C., Spoelstra, S.F., Driehuis, F., Anaerobic conservation of lactic acid to acetic acid and 1,2 propanediol by Lactobacillus buchneri. Applyed Environmental Microbiology, 2001, 67, 125-132.
Filya, I., Sucu, E., The effect of Bacterial Inoculants and Chemical preservative on the fermentation and aerobic stability of whole – crop cereal silages, Asian Australian Journal of Animal Sciences, 2007, 20 (3), 378-384
