Histomorphometric Study Regarding the Effect of Experimental Exposure of Carassius gibelio Species to Increasing Doses of Ethylenediaminetetraacetic Acid(EDTA)
Keywords:
EDTA, freshwater fish, gill, histomorphometric study, ovary, small intestineAbstract
The aim of this paper is to show the preliminary results regarding some histomorphometrical changes induced by chronic exposure of Prussian carp specimens to increasing doses of EDTA.
150 Prussian carps, 10-12 g of weight, collected from a local pond and acclimated for two weeks in laboratory conditions were housed in 60 L capacity glass aquariums (30 individuals/aquarium) and divided into four groups: a control group maintained in EDTA-free freshwater and others three groups receiving 0.05 g EDTA/L water, 0.1 g EDTA/L water and 0.15 g EDTA/L water respectively.
Tissue samples (gill, small intestine and ovary) were processed and microscopically examined at the end of a 21 days experimental period. A specific QuickPHOTO Micro 2.2 software has been used for the histomorphometric study.
Gill lamellae length, intestinal villi height and chorion thickness of ovaries follicles suffered EDTA dose-dependent histomorphometrical changes.
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