Sorghum Bagasse as Substrate for Cellulase Production by Submerged and Solid-State Cultures of Trichoderma
Keywords:
cellulases, sorgum bagasse, TrichodermaAbstract
Sweet sorghum bagasse was used as cellulosic substrate in submerged and solid-state cultures of Trichoderma for cellulase production. Submerged liquid cultures (SLC) were obtained by inoculation of Mandels media containing 1% cellulose with spores suspension of Trichoderma. Solid-state cultures (SSC) were carried out in Erlenmayer flasks, where the substrate was distributed 1 cm layers. Comparing the yields of cellulases produced by Trichoderma strains in the systems applied in this study, using as substrate sorghum bagasse, we found the solid-state cultures as the system to produce the highest cellulase yields. The local strain of T. viride CMIT3.5. express high productivity in SSC system in laboratory conditions. The cellulolytic enzymes have maximum activity at 50oC, pH 4,8. The results recommend solid-state cultures of Trichoderma on sorghum bagasse as systems for producing cellulolytic products with higher activity than submerged cultures of Trichoderma on the same substrate.
References
Deb, U. K., Bantilan, M.C.S., Roy, A.D., Parthasararhy Rao, P., Global Sorghum Production Scenario, Broshure, http://oar.icrisat.org.
Lynd, L.R., Overview and evaluation of fuel ethanol from cellulosic biomass: technology, economics, the environment, and policy, Ann. Rev. Energy Environ., 1996, 21 403–465.
Vintila, T., Dragomirescu M., Vintila D., Croitoriu V., Hydrolysis of three types of lignocelluloses from agriculture using commercial enzymes and culture filtrate of Trichoderma viride, Journal of Biotechnology, 2010, vol. 150S.
Sanchez, O.J., Cardona C.A., Trends in biotechnological production of fuel ethanol from different feedstocks, Bioresource Technology, 2007, xxx 1 – 26.
Diguţa, C., Jurcoane, Ş. Israel– Roming, F., Brule, M., Mukengele, M., Lemmer, A., Oechsner, H., Studies concerning enzymatic hydrolysis of energy crops, Roumanian Biotechnological Letters, 12 (2), 3203, 3207 (2007).
Sukumaran, R. K., Singhania, R. R., Mathew, G. M., Pandey, A., Cellulase production using biomass feed stock and its application in lignocellulose saccharification for bio-ethanol production, Renewable Energy 34 (2009) 421-424.
Wingren, A., Galbe M., Zacchi G., Techno-economic evaluation of production ethanol from softwood: comparison of SSF and SHF and identification of bottlenecks, Biotechnology Progress, 2003, vol. 19, no.4, 1109-1117.
Sassner, P., Zacchi G., Integration option for high energy efficiency and improved economics in a wood-to-ethanol process, Biotechnology for Biofuels, 2008, vol. 1, article 4.
Barta, Z., Kovacs K., Reczey K., Zacchi G., Process design and economics of on-site cellulase production on various carbon sources in a softwood-based ethanol plant, Enzyme Research, 2010, article ID 734182.
Merino, S.T., Cherry J., Progress and challenges in enzyme development for biomass utilization, in Biofuels, vol. 108 of Advances in Biochemical Engineering/Biotechnology, 2007, pp. 95-120.
Mohammad, J. T., Keikhosro K., Pretreatment of lignocellulosic Wastes to Improve Ethanol and Biogas Production. International Journal of Molecular Sciences, 2008, 9(9) 1621,1651,
Vintila T., Dragomirescu M., Croitoriu V., Vintila C., Barbu H., Sand C., Saccharification of lignocellulose - with reference to Miscanthus - using different cellulases, Romanian Biotechnological Letters., 15(4), 5498, 5504 (2010).
Ghose, T.K. - International union of pure and applied chemistry – Applied chemistry division – Commission on biotechnology, Measurement of cellulase activities, in Pure and Applied Chem., 1987, vol. 59, No.2, 257-268.
Vintila, T., Croitoriu, V., Dragomirescu, M., The Effects of Bioprocess Parameters on Cellulase Production with Trichoderma viride CMIT35, Scientifical Papers Animal Sciences and Biotechnologies, 2010, Vol. 43 (1),.
Trulea, A., Vintila T., Pop G., Dragomirescu M., Effects of Bioprocess Parameters on Production of Cellulase using Miscanthus as Substrate, Scientifical Papers Animal Sciences and Biotechnologies, 2013, 46 (1).
El-Nawawy, A.S., Microbial biomass production from organic solid substrates. In: Solid Substrate Cultivation, H.W. Doelle, D.A. Mitchell, E.R. Carlos, Ed. Elsevier, 1992, pp. 247-265.
