The Analysis of the MspI Polymorphism within Exon 3 of the Calpastatin Gene in Slovak Simmental Cattle
Keywords:
calpastatin gene, DNA, fertility, PCR-RFLP, Slovak Simmental cattleAbstract
Calpastatin has important function in the tenderization process. The SNP polymorphisms in the calpastatin gene such as UoG-CAST (intron 5) and CAST-T1 (3´UTR region) are used as markers in commercial test for prediction of animals with tenderness meat. The others research of the calpastatine gene confirmed the impact of a SNP polymorphism in exon 3 on fertility and longevity in dairy cattle, too. The aim of this study was to analyse the population of 113 animals of Slovak Simmental (42 bulls and 71 cows) for the missense SNP polymorphism in exon 3. Bovine genomic DNA was isolated from sperm and blood by commercial kit. The SNP CAST c.283 C>T was detected by PCR-RFLP method with restriction endonuclease MspI. The favourable allele C was detected by two restriction fragments 135 bp and 173 bp and the allele T with the one 308 bp fragment. In the analyzed population of Slovak Simmental cattle were detected the following frequency of alleles and genotypes for the SNP c.283 C>T of the CAST gene. Frequencies of allele C and allele T were 0.6460 and 0.3540 and frequencies of genotypes were 0.4336 (genotype CC), 0.4248 (genotype CT) and 0.1416 (genotype TT).
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