Tetraploidy Determination in Rainbow Trout (Oncorhynchus mykiss) Based on Erythrocytes Dimensions

Authors

  • Ioan Bencsik Banat's University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 300645- Timişoara, Calea Aradului, 119, Timisoara, Romania
  • Nicolae Pacala Banat's University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 300645- Timişoara, Calea Aradului, 119, Timisoara, Romania
  • Gabi Dumitrescu anat's University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 300645- Timişoara, Calea Aradului, 119, Timisoara, Romania
  • Dorel Dronca anat's University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 300645- Timişoara, Calea Aradului, 119, Timisoara, Romania
  • Jana Stanculet SC Detergenti SA, 30057-Timisoara, Calea Stan Vidrighin, 5, Romania
  • Liliana Petculescu-Ciochina SC Detergenti SA, 30057-Timisoara, Calea Stan Vidrighin, 5, Romania
  • Liliana Boca Banat's University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 300645- Timişoara, Calea Aradului, 119, Timisoara, Romania

Keywords:

erythrocytes, heat shock, rainbow trout, tetrapoidy

Abstract

Tetraploidy induction at fish is characterized by modification of normal diploid chromosome set (2n) into tetraploid set (4n). Experiments were carried out on biological material from rainbow trout (Oncorhynchus mikiss) during the natural breeding season. Polyploidy was induced by exposing the eggs to heat shock. Blood smear was used as a technical method, to determine diploid and tetraploid status. Staining of blood smear was performed by Pappenhein method. The erythrocytes area and perimeter measurements done comparatively on tetraploid and diploid individuals may represent an indicator to determine the ploidy level of individuals. Erythrocytes area for tetraploid individuals is 2.18 times higher than at diploid individuals, and perimeter 1.45 times higher than in diploid individuals.

References

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Published

2023-09-05

Issue

Vol. 45 No. 1 (2012): Scientific Papers: Animal Science and Biotechnologies

Section

Animal Reproduction

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.