Genetic Polymorphisms of β-Lactoglobulin and α-s1-Casein Genes in Romanian Racka Sheep
Keywords:
Racka breed, milk proteins, genetic polymorphism, PCR-RFLP.Abstract
The increasingly use of genetic markers in artificial selection shows to be a successful strategy for the animal production systems, the outcome of applying such methods being a higher animal productivity and better quality of the products. The aim of our study was to analyse the polymorphism of β-lactoglobulin and α-s1-casein loci in a population of Racka sheep from Romania. In order to identify the specific alleles we used the PCR-RFLP technique. It is known that the different genotypes observed at these loci are correlated to milk quality, either in terms of the composition, or in terms of the technological properties. We identified three genotypes (AA, BB and AB), at β-lactoglobulin locus, while at α-s1-casein locus, all the analysed animals had nonA homozygous genotype. These molecular markers are useful for the characterisation of the sheep breeds, and their genotyping can contribute to a high quality artificial selection.
References
Savic, M., Trailovic, R., Petrujkic, B., Beckei, Z., Dimitrijevic, B., Dimitrijevic, V., Determining the value of Vlashko-Vitoroga Zackel sheep for the conservation process, Acta Veterinaria Beograd, 2013, 63(5-6), 621-629.
Bell, K., McKenzie, H.A., The whey proteins of ovine milk: β-lactoglobulins A and B, Biochimica et Biophysica Acta, 1967, 147, 123-134.
Erhardt, G., Evidence for a third allele at the β-lactoglobulin (β-Lg) locus of sheep milk and its occurrence in different breeds, Animal Genetics, 1989, 20, 197-204.
Amigo, L., Recio, I., Ramos, M., Genetic polymorphism of ovine milk proteins: its influence on technological properties of milk-a review, Dairy Journal, 2000, 10, 135–149.
Chessa, S., Bolla, P., Dario, C., Pieragostini, E., Caroli, A., 2003, Milk protein polymorphisms in the ovine breed Gentile di Puglia, Scienza e tecnica Lattiero-Casearia, 54, 191–198.
Elmaci, C., Oner, Y., Balcioglu, M., Genetic polymorphism of β-lactoglobulin gene in native Turkish sheep breeds, Biochemical Genetics, 2006, 44(7/8), 379-384.
Kevorkian, S., Manea, M.A., Georgescu, S.E., Dinischiotu, A., Costache, M., Genotyping of β-lactoglobulin gene in Karakul sheep breed, Scientific Papers Animal Sciences and Biotechnologies, 2008a, 41(1), 112-116.
Chianese, L., Garro, G., Mauriello, R., Laezza, P., Ferranti, P., Addeo, F., Occurrence of five alpha (s1)-casein variants in ovine milk, Journal of Dairy Research, 1996, 63(1), 49-59.
Recio, I., Ramos, M., Amigo, L., Study of the polymorphism of ovine αs1- and αs2-caseins by capillary electrophoresis, Journal of Dairy Research, 1997, 64(1), 525-534.
Giambra, I.J., Jager, S., Erhardt, G., Isoelectric focusing reveals additional casein variants in German sheep breeds, Small Ruminant Research, 2010, 90(1-3), 11-17.
Kevorkian, S., Manea, M.A., Georgescu, S.E., Dinischiotu, A., Costache, M., Genotyping of αS1-cazein gene in Karakul sheep breed, Lucrari Stiintifice, Universitatea de Stiinte Agricole si Medicina Veterinara "Ion Ionescu de la Brad Iasi", seria Medicina Veterinara, 2008b, 51(10), 79-81.
Sztankóova, Z., Matlova, V., Rychtarova, J., Genetic polymorphism at the milk protein genes (CSN1S1, CSN2, and CSN3) in the Czech Sumava and Wallachian sheep breeds, Grant Journal, 2012, 1(2), 63-66.
Georgescu, S.E., Isfan, N., Kevorkian, S.M., Rebedea, M., Costache, M., The correlation of production characteristics with the genetic variants of the encoding locus of β-lactoglobulin in three sheep breeds from Romania, Archiva Zootechnica, 2011, 14(1), 41–49.
Vatasescu-Balcan, R.A., Georgescu, S.E., Manea, M.A., Dinischiotu, A., Tesio, C.D., Costache, M., Identification of beta-lactoglobulin and kappa-casein genotypes in cattle, Scientific Papers Animal Sciences and Biotechnologies, 2007a, 40(1), 211–216.
Vatasescu, B.R.Al., Georgescu, S.E., Manea, M.A., Dinischiotu, A., Costache, M., Analysis of beta-lactoglobulin and kappa-casein genotypes in cattle, Archiva Zootechnica, 2007b, 10, 111-114.
Feligni, M., Parma, P., Aleandri, R., Greppi, G.F., Enne, G., PCR-RFLP test for direct determination of β-lactoglobulin genotype in sheep, Animal Genetics, 1998, 29, 460-477.
Pilla, F., Bevilacqua, C., Leroux, C., Fraghi, A., Martin, P., Genotyping of alphaS1-casein in sheep, Animal Genetics, 1998, 29, 472-473.
Recio, I., Fernandez-Fournier, A., Martin-Alvarez, P.J., Ramos, M., β-lactoglobulin polymorphism in ovine breeds: influence on cheese-making properties and milk composition, Lait, 1997, 77, 259-265.
Lourdes, A., Recio, I., Ramos, M., Genetic polymorphism of ovine milk proteins: its influence on technological properties of milk - a review, International Dairy Journal, 2000, 10, 135-149.
