Identification of Histological Changes in Liver and Evaluation of the Effect of Some Probiotics and Amino Acids on the Invasion of C. jejuni
Keywords:
C. jejuni, chicken broilers, liver, probiotic, histological changesAbstract
Poultry meat represents a worldwide food and can be a source of pathogen agents (Salmonella enterica spp., Escherichia coli, and Campylobacter jejuni). Therefore, identification of the best methods to reduce infections of these pathogens from poultry meat represents a critical aspect for producers. The purpose of this study was to identify the histological changes in liver and to evaluate the influence of some probiotic strains and of some amino acids in the invasion of Campylobacter jejuni. The biological material was represented by poultry broilers, the hybrid ROSS 308, which were randomly allocated in seven experimental groupss, of 10 individuals/group that were treated as follows: G0 (control); G1-L. paracasei CMGB 18 and L. rhamnosus CMGB 34 (0-42 days); G2-L. paracasei CMGB 18, L. rhamnosus CMGB 34, L. lactis CMGB 31 and L. lactis CMGB 32 (0-42 days); G3-L. paracasei CMGB 18 and L. rhamnosus CMGB 34 (35-42 days); G4-L. paracasei CMGB 18, L. rhamnosus CMGB 34, L. lactis CMGB 31 and L. lactis CMGB 32 (35-42 days); G5-L. paracasei CMGB 18, L. rhamnosus CMGB 34, L Treonine and DL Methionine (0-42 days); G6-L. paracasei CMGB 18, L. rhamnosus CMGB 34, L. lactis CMGB 31, L. lactis CMGB 32, L Treonine and DL Methionine (35-42 days). The microscopic aspects pointed out by us in this study suggest the pathogenicity of C. jejuni, marked by the appearance of inflammatory areas, with the presence of a perivascular and diffuse leukocytes infiltration and, also, presence of pyknotic nuclei. Moreover, the results show the beneficial effect of probiotic strains L. paracasei CMGB 18, L. rhamnosus CMGB 34, L. lactis CMGB 31 and L. lactis CMGB 32, with reduction of the changes caused by C.jejuni and the production of toxins, thus preventing the damage of hepatic cells. The most powerful effect was noticed when all the four probiotic strains were administrated, during the experiment of 0-42 days.
References
Raouf, Tareb, Marion, Bernardeau, Marielle, Gueguen, Jean-Paul, Vernoux, In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni, Journal of Medical Microbiology, 2013,62, 637–649
O’ Brien, S. J. & de Valk, H., Salmonella – ‘old’ organism, continued challenges! Euro Surveill, 2003, 8, 29–31.
Crushell, E., Harty, S., Sharif, F. & Bourke, B., Enteric Campylobacter: purging its secrets? Pediatr Res, 2004, 55, 3–12.
Blasé, M.J., Epidemiologic and clinical features of Campylobacter jejuni, J.Infect.Dis, 1997, 176, S103-105
Tauxe, R. V., Epidemiology of Campylobacter jejuni infections in the United States and other industralized nations. In Campylobacter jejuni: Current Status and Future Trends, pp. 9–19. Edited by I. Nachamkin, M. J. Blaser & L. S. Tompkins. Washington, DC: American Society for Microbiology, 1992
Taylor, D. N., Campylobacter infections in developing countries. In Campylobacter jejuni: Current Status and Future Trends, pp. 20–30. Edited by I. Nachamkin, M. J. Blaser & L. S. Tompkins. Washington, DC: American Society for Microbiology, 1992.
Allos, B.M., Campylobacter jejuni Infections: update on emerging issues and trends. Clin Infect Dis, 2001, 32, 1201–1206.
Stafford, R. J., Schluter, P. J., Wilson, A. J., Kirk, M. D., Hall, G., & Unicomb, L., Population-attributable risk estimates for risk factors associated with Campylobacter infection, Australia. Emerg Infect Dis, 2008, 14, 895–901.
Keita, Nishiyama, Yasuyuki, Seto, Kazuki, Yoshioka, Tsutomu, Kakuda, Shinji, Takai, Yuji, Yamamoto, Taka,o Mukai, Lactobacillus gasseri SBT2055 Reduces Infection by and Colonization of Campylobacter jejuni, PLOS ONE, 2014, 9(9), e108827.
Altekruse, S. F., Stern, N. J., Fields, P. I., Swerdlow, D. L., Campylobacter jejuni–an emerging foodborne pathogen. Emerg Infect Dis, 1999, 5, 28–35.
Hermans, D., Van Deun, K., Martel, A., Van Immerseel, F., Messens, W., et al., Colonization factors of Campylobacter jejuni in the chicken gut. Vet Res, 2011, 42, 82.
Tsang, R. S. W., The relationship of Campylobacter jejuni infection and the development of Guillain–Barre´ syndrome. Curr Opin Infect Dis, 2002, 15, 221–228.
Hannu, T., Kauppi, M., Tuomala, M., Laaksonen, I., Klemets, P. &Kussi, M., Reactive arthritis following an outbreak of Campylobacter jejuni infection. J Rheumatol, 2004, 31, 528–530.
Garcia Rodriguez, L. A., Ruigomez, A., & Panes, J., Acute gastroenteritis is followed by an increased risk of inflammatory bowel disease. Gastroenterology, 2006, 130, 1588–1594.
Marshall, J. K., Thabane, M., Garg, A. X., Clark, W.F., Salvadori, M., & Collins, S.M., Incidence and epidemiology of irritable bowel syndrome after a large waterborne outbreak of bacterial dysentery. Gastroenterology, 2006, 131, 445–450.
Young, K. T., Davis, L. M., Dirita, V. J., Campylobacter jejuni: molecular biology and pathogenesis. Nat Rev Microbiol, 2007, 5: 665–679.
Rosenquist, H., Nielsen, N. L., Sommer, H. M., Nørrung, B., Christensen, B. B., Quantitative risk assessment of human campylobacteriosis associated with thermophilic Campylobacter species in chickens. Int J Food Microbiol, 2003, 83, 87–103.
Ventura, M., O’Flaherty, S., Claesson, M.J., Turroni, F., Klaenhammer, T. R., van Sinderen, D., & O’Toole, P.W., Genome-scale analyses of health-promoting bacteria: probiogenomics. Nat Rev Microbiol, 2009, 7, 61–71.
FAO/WHO, Report of a Joint FAO/WHO Expert Consultation on Evaluation of Health, and Nutritional Properties of Probiotics in Food Including Powder Milk with Live Lactic Acid Bacteria (Córdoba: Foodand Agriculture Organizationof the United Nations, World Health Organization), 2001.
Lebeer, S., Vanderleyden, J. & De Keersmaecker, S. C. J., Genes and molecules of lactobacilli supporting probiotic action. Microbiol Mol Biol Rev, 2008, 72, 728–764.
Brancroft, J. D., Stevens A., Theory and Practice of Histological Techniques. 4th ed. Churchill-Livingstone, New York., 1996.
Walker, R. I., M. B. Caldwell, E. C. Lee, P. Guerry, T. J. Trust, and G. M. Ruiz-Palacios, Pathophysiology of Campylobacter enteritis, Microb. Rev., 1986, 50:81–94.
Guerra, Lina, Ximena Cortes-Bratti, Riccardo Guidi and Teresa Frisan, 2011. The Biology of the Cytolethal Distending Toxins. Toxins, 2011, 3, 172-190; doi:10.3390/toxins3030172
Zhongming Ge, David B. Schauer, James G. Fox, In vivo virulence properties of bacterial cytolethal-distending toxin. Cellular Microbiology, 2008, 10(8), 1599–1607.
Thelestam, M., Frisan, T., Cytolethal distending toxins. In The Comprehensive Sourcebook of Bacterial Protein Toxins; Alouf, J., Popoff, M., Eds.; CPL Press: Newbury, UK, pp. 448–467, 2006.
Cortes-Bratti, X., Chaves-Olarte, E., Lagergård, T., Thelestam, M., Cellular internalization of cytolethal distending toxin from Haemophilus ducreyi. Infect. Immun, 2000, 68, 6903–6911.
Guerra, L., Nemec, K. N., Massey, S.; Tatulian, S.A., Thelestam, M., Frisan, T., Teter, K., A novel mode of translocation for cytolethal distending toxin. Biochim. Biophys. Acta, 2009,1793, 489–495.
Nishikubo, S., Ohara, M., Ueno, Y., Ikura, M., Kurihara, H., Komatsuzawa, H., Oswald, E., Sugai, M., An N-terminal segment of the active component of the bacterial genotoxin cytolethal distending toxin B (CDTB) directs CDTB into the nucleus. J. Biol. Chem., 2003, 278, 50671–50681.
Cortes-Bratti, X., Karlsson, C., Lagergard, T., Thelestam, M., Frisan, T., The Haemophilus ducreyi cytolethal distending toxin induces cell cycle arrest and apoptosis via the DNA damage checkpoint pathways. J. Biol. Chem., 2001, 276, 5296–5302.
Shenker, B.J., McKay, T., Datar, S., Miller, M., Chowhan, R., Demuth, D., Actinobacillus actinomycetemcomitans immunosuppressive protein is a member of the family of cytolethal distending toxins capable of causing a G2 arrest in human T cells. J. Immunol., 1999, 162, 4773–4680.
Comayras, C., Tasca, C., Peres, S.Y., Ducommun, B., Oswald, E., de Rycke, J., Escherichia coli cytolethal distending toxin blocks the HeLa cell cycle at the G2/M transition by preventing cdc2 protein kinase dephosphorylation and activation. Infect. Immun., 1997, 65, 5088–5095.
.Varankovich, N. V., Nickersonand, M. T., Korber, D., R., Probiotic-based strategies for therapeutic and prophylactic use against multiple gastrointestinal diseases, Frontiers in Microbiology, 2015, Vol. 6, Article 685, doi: 10.3389/fmicb.2015.00685.
Collado, M. C., Surono, I., Meriluoto, J. & Salminen, S., Indigenous dadih lactic acid bacteria: cell-surface properties and interactions with pathogens. J Food Sci, 2007, 72, M89–M93.
Foligné, B., Dewulf, J., Breton, J., Claisse, O., Lonvaud-Funel, A., and Pot, B., Probiotic properties of non-conventional lactic acid bacteria: immunomodulationby Oenococcusoeni. Int. J. Food Microbiol., 2010, 140, 136–145. doi: 10.1016/j.ijfoodmicro.2010.04.007
Konieczna, P., Ferstl, R., Ziegler, M., Frei, R., Nehrbass, D., Lauener, R.P., Immunomodulation by Bifidobacterium infantis 35624 in the murine lamina propria requires retinoic acid-dependent and independent mechanisms. PLoSONE 8:e62617. doi:10.1371/journal.pone.0062617, 2013.
Schachtsiek, M., Hammes, W.P., Hertel, C., Characterization of Lactobacillus coryniformis DSM 20001T surface protein Cpf mediating co-aggregation with and aggregation among pathogens. Appl Environ Microbiol, 2004, 70, 7078–7085.
Wine, E., Gareau, M. G., Johnson-Henry, K. & Sherman, P. M., Strain-specific probiotic (Lactobacillus helveticus) inhibition of Campylobacter jejuni invasion of human intestinal epithelial cells. FEMS Microbiol Lett, 2009, 300, 146–152.
Chaveerach, P., Lipman, L. J., & van Knapen, F., Antagonistic activities of several bacteria on in vitro growth of 10 strains of Campylobacter jejuni/coli. Int J Food Microbiol, 2004, 90, 43–50.
Ding, W., Wang, H., & Griffiths, M.W., Probiotics downregulate flaA sigma28 promoter in Campylobacter jejuni. J Food Protect, 2005, 68: 2295–2300.
Blaser, M., Duncand, J., Warreng, H., Wang, W. L. L, Experimental Campylobacter jejuni infection of adult mice. Infection and Immunity, 1983, 39, 908-916.
Kita, Eiji, Noriaki Katsui, Kazuyuki Nishi, Masashi Emot0, Yasuo Yanagase, Shuzo Kashibai, Hepatic Lesions in Experimental Cmpylobacter jejuni Infection of Mice. Journal of General Microbiology, 1986, 132, 3095-3103.
