Intestinal Bacterial Community of the Apis Melifera Carpatica Honey Bee Workers, Depending on Season and Area
Keywords:
bacteria colony, Gram-positive, Gram-negative, intestinal micro-flora, medium of cultureAbstract
It is important to know that the intestinal flora of most organisms play a decisive role in nutrient assimilation or diseases defending action and honeybees are not an exception. The importance and composition of the intestinal micro-flora define the health and growth of honeybees. For this study were been collected as far as 10 honeybee workers from each area, from three different areas: Brasov County, near Perşani Mountains, Timiş County, from plane area and Arad County, from hillside area, from beekeepers, both in June and December. First was been detected the total number of bacteria contained in honeybees intestine samples through dilution method. The largest number of countered bacteria colonies was obtained from Timiş County (2946 colonies/millilitre) in December. The smallest number of countered bacteria colonies was obtained in Braşov County (527 colonies/millilitre) in June. After the growing colonies of bacteria from studied samples on special mediums of culture have been detected many species, Gram-positive including Bacillus spp., Streptococcus spp., and Gram-negative including Escherichia spp., Proteus, Citrobacter, Pseudomonas. Also have been detected Lactobacillus strains.
References
Reese Halter, The incomparable honeybee and the economics of pollination, Ed. Rocky Mountains Books, ISBN1926855647, 2011, p. 45-76.
Jeyaprakash A., Hoy, M.,A., Allsop M.,H., Bacterial diversity in worker adults of Apis meliferacapensis and Apis melifera scutellata, (Insecta: Hymenoptera) assessed using 16S rRNA sequences, Journal of Invertebrates Pathology, 2003, 84, 96-103.
Gilliam M., Identification and roles of non-pathogenic microflora associated with honey bees, FEMS Microbiol. Lett., (1997), 155, 1-10.
Gilliam M., Valentine D., K., Bacteria isolated from intestinal contents of foraging worker honey bees Apis melifera: the genus Bacillus, J. Invertebr. Pathol., 1976, 28, 275–276.
Lederberg J., McCray A., T., 'Ome Sweet 'Omics—a genealogical treasury of words. Scientist. 2001, 15, 8.
Madigan, Michael T., Brock biology of microorganisms (13th ed.). San Francisco: Benjamin Cummings, ISBN 9780321649638, 2012.
Mohr K., I, Tebbe C., C., Diversity and phylotype consistency of bacteria in the guts of three species (Apoidea) at an oilseed rape field, Environ. Microbiology, 2006, 8, 258-272.
Gillian M., Morton H., L., Bacteria belonging to the genus Bacillus isolated from honey bees, Apis melifera fed 2,4-D and antibiotics, Apidologie, 1978, 9, 213-222.
Gillian M., Taber S., Lorenz B., I., Prest D., B., Factors affecting development of chalkbrood disease in colonies of honey bees, Apis melifera, fed pollen contaminated with Ascosphera apis, Journal Invertebr. Pathology, 1988, 52, 314-325.
Williams D., L., Review. A veterinary approach to the European honey bee (Apis melifera), Veterinary Journal, 2000, 160, 61-73.
Evans G., D., Amstrong T., N., Antagonistic interactions between honey bee bacterial symbionts and implications for disease, BMC Ecol., 2006, 6, 4-7.
Babendreier D., Joller D., Romeis J., Bigler F., Widmer F., Bacterial community structures in honey bee intestines and their response to two insecticidal proteins, FEMS Microbiol. Ecology, 2007, 59, 600-610.
Dillon R., J., Dillon V., M., The gut bacteria of insects, Annu. Rev. Entomology, 2004, 49, 71-92.
