Cyto-Histological Manifestations of the Apoptotic Process in the Prepubescent Mouse Ovary (14-17 days)
Keywords:
apoptosis, follicles, morphological changes, prepubescent mouse ovaryAbstract
Follicular atresia is a process commonly encountered at ovarian level that limits the number of ovulations and, respectively, the reproductive potential of a female. Ovarian cells’ death is essential for maintaining homeostasis of this organ and is based on an apoptotic mechanism that ensures selection of the dominant follicle and the disappearance of follicles in excess. The present study evaluates the incidence of morphological changes specific for this process in the prepubescent mouse ovary by applying optical microscopy techniques. Microscopic analysis was performed on sections of ovarian tissue from mice females’ line NMRI aged 14-17 days. The results show, at this age, significant morphological changes at cellular level, specific for apoptotic process, as condensation and fragmentation of the genetic material, vacuolization of cell cytoplasm, destabilization of cell adhesion junctions and appearance of wide intercellular spaces, invasion of created spaces with infiltrated leukocytes, formation of apoptotic bodies.
References
Amsterdam, A., Keren-Tal, I., Aharoni, D., Dantes, A., Land-Brecha, A., Rimon, E., Sasson, R., Hirsh, L, Steroidogenesis and apoptosis in the mammalian ovary, Steroids, 2003, 68(10), 861-867.
Vaskivuo, T. E., Tapanainen, J. S., Apoptosis in the human ovary, Reprod. Biomed. Online, 2003, 6, 24-35.
Yakovlev, A. G., Wang, G., Stoica, B. A., Boulares, H. A., Spoonde, A. Y., Yoshihara, K., Smulson, M. E., A role of the Ca2+/Mg2+-dependent endonuclease in apoptosis and its inhibition by poly (ADP-ribose) polymerase, Department of Biochemestry, Georgetown University, Washinton, 2007.
Petculescu-Ciochină, L., Păcală, N., Dumitrescu, G., The Action of Bcl-2 Apoptotic Family Proteins and Caspases in Mediating Follicle Atresia in Adult Mouse, Scientific Papers: Animal Science and Biotechnologies, 2011, 44(1), 376-379.
Petculescu-Ciochină, L., Dumitrescu, G., Păcală, N., Voia, O. S., Popescu, R., Filimon, M. N., IHC-P analysis of apoptotic process, in adult mouse ovary, by highlighting the Bcl-2 and Bax proteins, Romanian Biotechnological Letters, 2014, 19(2), 9119-9127.
Hussein, M.,R., Apoptosis in the ovary molecular mechanisms, Hum. Reprod., 2005, 11(2), 162-168.
Wu, J., Zhang, L., Wang, X., Maturation and apoptosis of human oocytes in vitro are age-related, Fertil. Steril., 2000, 74, 1137-1141.
Fulton, N., Martins Da Silva, S. J., Bayne, R. A., Anderson, R. A., Germ cell proliferation and apoptosis in the developing human ovary, J. Clin. Endocrinol. Metab., 2005.
Mihm, M., Austin, E. J., Good, T. E. M., Ireland, J. L. H., Knight, P. G., Roche, J. F., Ireland, J. J., Identification of potenţial intrafollicular factors involved in selection of dominant follicles in heifers, Biol. Reprod., 2000, 63, 811-819.
Austin, E. J., Mihm, M., Evans, A. C. O., Knight, P. G., Ireland, J. L. H., Ireland, J. J., Roche, J. F., Alteration in intraffolicular regulatory factors and apoptosis during selection of follicles in the first folicular wave of the bovine estrous cycle, Biol. Reprod., 2001, 64, 839-84
Ginther, O. J., Beg, M. A., Kot, K., Meira, K., Bergfelt, D. R., Associated and independent comparisons between the two largest follicles preceding follicle deviation in cattle, Biol. Reprod., 2003, 68, 524-529.
Quirk, S. M., Cowan, R. G., Harman, R. M., Hu, C. L., Porter, D. A., Ovarian follicular growth and atresia: the relation ship between cell proliferation and survival, Cornell University, Ithaca, NY 14853, 2003.
Yu, S. Y., Sui, H. S. Han, S. B., Li, W., Luo, M. J., Tan, J. H., Apoptosis in granulosa cells during follicular atresia: relationship with steroids and insulin- like growth factors, Cell Research, 2004, 14, 341-346.
Santos, H. B., Moro, L. Y. Bazzoli, N., Rizzo, E., Relationship among follicular apoptosis, integrin beta1 and collagen type IV during early ovarian regression in the teleost Prochilodus argenteusafter induced spawning, Cell Tissue Res., 2008, 332(1), 159-170.
Svanberg, B., Apoptosis- the cellular mechanism of rat ovarian follicular atresia, Department of Physiology, Göteborg University, Sweden, 2000.
Berger, F., L’apoptose ou mourt cellulaire programeé, Biologie cellulaire, Universite Joseph Fourier, Faculté de Médecine de Grenoble, 2007.
Johnson, A. L., Bridgham, J. T., Caspase- mediated apoptosis in the vertebrate ovary, Department of Biological Sciences, University of Notre Dame, IN 46556, USA, 2002.
Takagi, K., Yamada, T., Miki, Y., Umegaki, T., Nishimura, M., Sasaki, J., Histological observation of the development of follicles and follicular atresia in immature rat ovaries, Acta. Med. Okayama, 2007, 61 (5), 283-298.
Üçüncü Sema İ., Çakici, Ö., Atresia and Apoptosis in Preovulatory Follicles in the Ovary of Danio rerio (Zebrafish), Turkish Journal of Fisheries and Aquatic Sciences, 2009, 9, 215-221.
Dumitrescu, Gabi, Anatomie-Histologie-Embriologie, Ed. Mirton, Timişoara, 2007.
Cecconi, S., Ciccarelli, C., Barberi, M., Macchiarelli, G., Canipari, R., Granulosa cell-oocyte interactions, Eur. J. Obstet. Gynecol., 2004, 115 (Suppl 1), pp. S19-S22.
Hassegawa, J., Yanaihara, A., Iwasaki, S., Otsuka, Y., Negishi, M., Akahane, T., Reduction of progesterone receptor expression in human cumulus cells at the time of oocyte collection during IVF is associated with good embryo quality, Hum. Reprod., 2005, 20, 2194-2200.
