Effects of Alpha-Lipoic Acids on Sperm Membrane Integrity during Liquid Storage of Boar Semen
Keywords:
alpha-lipoicacid, liquid storage semen, membrane integrity, flow cytometerAbstract
Preliminary studies have shown that sperm membrane from swine shows high sensitivity to cryopreservation process, causing a dramatic reduction in sperm quality. This has been attributed to the production of reactive oxygen species that cause lipid peroxidation in sperm membranes. The aim of the present study was to minimize the oxidative attack by adding different concentration of alpha-lipoic acid into the sperm liquid storage at 17ºC for 7 days. Freshly ejaculated boar semen was diluted with Beltsville Thawing Solution (BTS) and supplemented with 5 levels of alpha-lipoic acid (0.015, 0.02, 0.05, 0.1, 0.15 mmol/ml). The membrane integrity was evaluated at days 0, 1, 3, 5 and 7 of liquid preservation, using flow cytometer FACSCanto II (BD Biociencias) systems. The experiment indicate that supplementation of alpha-lipoic acid to the semen liquid storage extender improve sperm membrane.
References
Bailey JL, Lessard C, Jacques J, Breque C, Dobrinski I, Zeng W, Galantino-Homer HL., Cryopreservation of boar semen and its future importance to the industry. Theriogenology. 2008, 70 (8), 1251-1259;
David Martin-Hidalgo, Ana Hurtado de Llera, Heiko Henning, Ulrike Wallner, Dagmar Waberski,Maria J. Bragado, Maria C. Gil, Luis J. Garcia-Marin, The Effect of Resveratrol on the Quality of Extended Boar Semen During Storage at 17ºC, Journal of Agricultural Science; 2013, Vol. 5, No. 8, 231-242;
Wagner, H.G. and Thibier, M. World statistics for artificial insemination in small ruminants and swine. Proc 14th ICAR, Stockholm, Sweden. 2000. vol. 2, 15:3;
Hernandez M, Roca J, Gil MA, Vazquez JM, Martinez EA., Adjustments on the cryopreservation conditions reduce the incidence of boar ejaculates with poor sperm freezability. Theriogenology. 2007,67(9), 1436-1445;
Holt, W.V.. Basic aspects of frozen storage of semen. Anim. Reprod. Sci. 2000b 62:3–22.
Ibrahim SF, Osman K, Das S, et al. A study of antioxidant effect of alpha−lipoic acids on sperm quality. Clinics 2008, 63:545−550;
Polge, C. Artificial insemination in pigs. Vet. Rec. 1956. 68: 62-76;
Johnson, L. A. Fertility results using frozen boar spermatozoa: 1970 to 1985. In L. A. Johnson, K. Larsson (Ed.), Deep freezing of boar semen. Uppsala, Swedish Univ Agric Sci: 1985.199-222;
Safranski T.J., J.J. Ford, G.A. Rohrer and H.D. Guthrie, Plenary Contribution to International Conference on Boar Semen Preservation 2011. Genetic Selection for Freezability and its Controversy with Selection for Performance. Reproduction in Domestic Animal, 2011,46 (Suppl. 2), 31–34;
Martinez-Alborcia MJ, Valverde A, Parrilla I, Vazquez JM, Martinez EA, Detrimental Effects of Non-Functional Spermatozoa on the Freezability of Functional Spermatozoa from Boar Ejaculate. PLoS ONE 2012,7(5);
Watson, P. F. The causes of reduced fertility with cryopreserved semen. Anim Reprod. Sci. 2000. 60-61:481-492;
Rodriguez-Martinez H. and Margareta Wallgren, Advances in boar semen cryopreservation. Veterinary Medicine International, 2011, doi:10.4061/2011/396181;
Heriberto Rodriguez-Martinez. Cryopreservation of Porcine Gametes, Embryos and Genital Tissues: State of the Art. In: Current Frontiers in Cryobiology, Igor I. Katkov (Ed.), 2012, ISBN: 978-953-51-0191-8, InTech, Available from: http://www.intechopen. com/books/current-frontiers-in-cryobiology/cryopreservation-of-pig-spermatozoa- oocytes-and-embryos-state-of-the-art;
Kim SH, Yu DH, Kim YJ., Apoptosis-like change, ROS, and DNA status in cryopreserved canine sperm recovered by glass wool filtration and Percoll gradient centrifugation techniques. Anim Reprod Sci., 2010, 119:106-14;
Ibrahim SF, Osman K, Das S, et al. A study of antioxidant effect of alpha−lipoic acids on sperm quality. Clinics, 2008, 63:545−550;
Siti Fatimah Ibrahim, Farah Hanan Fathihah Jaffar, Khairul Osman, Syarifah Faezah Syed Mohamed, Chew Fang Nang, Nur Hilwani Ismail, Mohd Iswadi Ismail, Bull spermatozoa motility: optimization of Coenzyme Q10 and Alpha-Lipoic acid concentration, IIOABJ; 2011, Vol. 2; Issue 5, 8–1;
Vázquez J.M., Martinez E.A., Martinez P., Garcia-Artiga C., Roca J. - Hypoosmotic swelling of boar spermatozoa compared to other methods for analysing the sperm membrane. Theriogenology 1997 47, p: 913-922;
Roca J., Parrilla I., Rodriguez-Martinez H., Gil M.A., Cuello C., Vazquez J.M., Martinez E.A., Approaches towards efficient use of boar semen in the pig industry. Reprod Domest Anim. 2011, 46(2), 79-83.
