Identification of DNA Fingerprint Pattern Variability of Known Retrotrasposons in Plantago lanceolata L.
Keywords:
Plantago lanceolata, iPBS, retrotransposonsAbstract
Plantago lanceolata L. belongs to important medical plants and is numerously represented in the plant communities of meadows, fields, pastures and permanent grasslands. Here, analysis of the presence of selected transponable elements sequences in the genome of Plantago lanceolata, L were performed. Retrotransposon are an inevitable part of plant genomes are one of the DNA markers that possess good amplification profiles useful for variability analysis. Known sequences of retrotransposons Tst-1, Cassandra, BARE-1, FaRe-1 and HACRE1 were used for a total of 10 specific primer designation and IRAP analysis as well as selected iPBS markers. The presence of the known retrotransposon sequences were confirmed by PCR analysis with different polymorphic potential. A total of 22 different iPBS markers were used from which 15 were confirmed to amplify polymorphic fingerprint patterns. Comparing obtained fingerprint profiles, iPBS markers resulted in more abundant profiles with higher potential to detect intraspecific differences for Plantago lanceolata L.
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