Thuja occidentalis Essential Oil: in vitro and in situ Antibacterial Potential against Phytopathogenic Bacteria and Insecticidal Activity
Keywords:
Thuja occidentalis essential oil, phytopathogenic bacteria, antibacterial and insecticidal effectAbstract
Thuja occidentalis essential oil (TOEO) was evaluated for antibacterial activity against phytopathogenic bacteria in vitro (disc diffusion) and in situ (fruit/vegetable models), and for insecticidal activity against Megabruchidius dorsalis. Tested bacteria were Xanthomonas arboricola, Pectobacterium carotovorum, Pseudomonas syringae and Agrobacterium radiobacter. In vitro, the largest inhibition zone was observed for X. arboricola (11.67 mm), while the smallest was for A. radiobacter (7.67 mm). In situ, the strongest effect occurred at 62.5 μg/mL on strawberry against carotovorum (78.96% inhibition) and on beetroot against A. radiobacter (79.56% inhibition). Insecticidal tests showed increasing mortality of M. dorsalis with rising TOEO concentration, reaching 100% at 100%. Within the tested ranges and models, TOEO exhibited measurable antibacterial and insecticidal effects. Findings are limited to the employed matrices, concentrations and incubation conditions, and do not imply broader applicability without additional sensory/toxicity and shelf-life testing.
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