Plants and Phytocompounds as Natural Defenders Against Mycotoxin Contamination in Agriculture
Keywords:
antifungal activity, mycotoxins, phytocompounds, sustainable agricultureAbstract
Plants play a key role in preventing mycotoxin contamination in agriculture through both direct antifungal effects and indirect environmental modulation. This review explores how specific phytocompounds - especially those from aromatic and medicinal plants - can inhibit fungal growth and suppress mycotoxin production. Compounds such as phenolics, flavonoids, and essential oils from oregano (Origanum vulgare), thyme (Thymus vulgaris), and rosemary (Rosmarinus officinalis) have demonstrated both antifungal and anti-mycotoxigenic properties. The role of phytoalexins, synthesized by plants in response to pathogenic attacks, is also addressed as a rapid natural defense mechanism. By synthesizing recent experimental data, this review highlights the potential of plant-derived compounds to be integrated into sustainable agricultural practices as natural alternatives to synthetic fungicides. Although promising, the application of phytocompounds requires careful assessment of their efficacy, stability, and potential limitations under field conditions.
References
Awuchi, C. G., Ondari, E. N., Ogbonna, C. U., Upadhyay, A. K., Baran, K., Okpala, C. O. R., Korzeniowska, M., & Guiné, R. P. F. Mycotoxins Affecting Animals, Foods, Humans, and Plants: Types, Occurrence, Toxicities, Action Mechanisms, Prevention, and Detoxification Strategies—A Revisit. Foods, 2021 Jun 3; 10(6):1279. doi: 10.3390/foods10061279.
Perincherry, L., Lalak-Kanczugowska, J., & Stepien, L. Fusarium-Produced Mycotoxins in Plant-Pathogen Interactions. Toxins (Basel), 2019; 11(11):664. doi: 10.3390/toxins11110664.
Poroșnicu, I., Ailincăi, L.-I., Mareș, M. (2023). The world of mycotoxins – A systematic review. Scientific Works. Series C. Veterinary Medicine, Vol. LXIX, Issue 1, ISSN 2065-1295, pp. 189-198.
Thipe, V. C., Bloembaum, P., Khoobchandani, M., Karikachery, A. R., Katti, K. K., & Katti, K. V. Green Nanotechnology: Nanoformulations Against Toxigenic Fungi to Limit Mycotoxin Production. In: Rai, M., & Abd-Elsalam, K. (Eds.), Nanomycotoxicology: Treating Mycotoxins in the Nano Way. Elsevier, 2020; pp. 155–188.
Celik, K. Nanomycotoxicology. Elsevier, 2020. The Efficacy of Mycotoxin-Detoxifying and Biotransforming Agents in Animal Nutrition; pp. 271–284.
Iqbal, N., Riyazuddin, R., Nauman, M., Czékus, Z., Tahir Hayat, M., Poór, P., & Ördög, A. Role of Plant Defence System in Crop Protection Against Fusarium Pathogens. IntechOpen, 2024. doi: 10.5772/intechopen.1004924.
Haque, M. A., Wang, Y., Shen, Z., Li, X., Saleemi, M. K., & He, C. Mycotoxin Contamination and Control Strategy in Human, Domestic Animal and Poultry: A Review. Microbial Pathogenesis, 2020; 104095. doi: 10.1016/j.micpath.2020.104095.
Prakash, B., Kumar, A., Singh, P. P., & Songachan, L. S. Antimicrobial and Antioxidant Properties of Phytochemicals: Current Status and Future Perspective. In: Prakash, B., Kumar, A., Singh, P. P., & Songachan, L. S. (Eds.), Functional and Preservative Properties of Phytochemicals. Elsevier, 2020; pp. 1–45.
Meng, D., Garba, B., Ren, Y., Yao, M., Xia, X., Li, M., & Wang, Y. Antifungal Activity of Chitosan Against Aspergillus ochraceus and Its Possible Mechanisms of Action. International Journal of Biological Macromolecules, 2020; 158:1063–1070. doi: 10.1016/j.ijbiomac.2020.04.213.
Kavitha, K., Vijaya, N., Krishnaveni, A., Arthanareeswari, M., Rajendran, S., Al-Hashem, A., & Subramania, A. Nanomaterials for Antifungal Applications. In: Nanotoxicity, 2020; pp. 385–398.
Makhuvele, R., Naidu, K., Gbashi, S., Thipe, V. C., Adebo, O. A., & Njobeh, P. B. The Use of Plant Extracts and Their Phytochemicals for Control of Toxigenic Fungi and Mycotoxins. Heliyon, 2020 Oct 22; 6(10):e05291. doi: 10.1016/j.heliyon.2020.e05291.
Redondo-Blanco, S., Fernandez, J., Lopez-Ibanez, S., Miguelez, E. M., Villar, C. J., & Lombo, F. Plant Phytochemicals in Food Preservation: Antifungal Bioactivity—A Review. Journal of Food Protection, 2019; 83(1):163–171. doi: 10.4315/0362-028X.JFP-19-163.
Adebo, O. A., & Gabriela Medina-Meza, I. Impact of Fermentation on the Phenolic Compounds and Antioxidant Activity of Whole Cereal Grains: A Mini Review. Molecules, 2020; 25(4):927. doi: 10.3390/molecules25040927.
Lahlou, Y., Rhandour, Z., Amraoui, B. E., & Bamhaoud, T. Screening of Antioxidant Activity and the Total Polyphenolic Contents of Six Medicinal Moroccan Plants Extracts. Journal of Materials and Environmental Science, 2019; 10:133–1348.
Maj, W., Pertile, G., & Różalska, S. Comprehensive Antifungal Investigation of Natural Plant Extracts Against Neosartorya spp. (Aspergillus spp.) of Agriculturally Significant Microbiological Contaminants and Shaping Their Metabolic Profile. Scientific Reports, 2024; 14:8399. https://doi.org/10.1038/s41598-024-58791-4.
Loi, M., Paciolla, C., Logrieco, A., & Mule, G. Plant Bioactive Compounds in Pre- and Post-Harvest Management for Aflatoxins Reduction. Frontiers in Microbiology, 2020; 11:243. doi: 10.3389/fmicb.2020.00243.
Abdel-Fattah, S. M., Abosrea, Y. H., Shehata, F. E., Flourage, M. R., & Helal, A. D. The Efficacy of Thyme Oil as Antitoxicant of Aflatoxin(s) Toxicity in Sheep. Journal of American Science, 2010; 6:948–960.
Kocic-Tanackov, S., Dimic, G., Jaksic, S., Mojovic, L., Djukic-Vukovic, A., Mladenovic, D., & Pejin, J. Effects of Caraway and Juniper Essential Oils on Aflatoxigenic Fungi Growth and Aflatoxins Secretion in Polenta. Journal of Food Processing and Preservation, 2019; 43.
Poroșnicu, I., Ailincăi, L.-I., Postolache, A.-N., Neculai-Văleanu, S., Ariton, M.-A., Mareș, M. (2024). Evaluation of the health status of dairy cows during a mycotoxin screening of feed in a farm from North-East Romania. Scientific Papers. Series D. Animal Science, Vol. LXVII, No. 2, pp. 388-398.
Mossini, S. A., Arrotéia, C. C., & Kemmelmeier, C. Effect of neem leaf extract and neem oil on Penicillium growth, sporulation, morphology and ochratoxin A production. Toxins (Basel), 2009; 1(1): 3–13.
Zadravec, M., Markov, K., Lešić, T., Frece, J., Petrović, D., & Pleadin, J. Biocontrol Methods in Avoidance and Downsizing of Mycotoxin Contamination of Food Crops. Processes, 2022, 10, 655. https://doi.org/10.3390/pr10040655.
Mulaudzi, R. B. Mycotoxins of Medicinal Plants and Human Health. Elsevier, 2019:524–530.
Rashed, A. B., Rathi, D. G., Ahmad Nasir, N. A. H., & Abd Rahman, A. Z. Antifungal Properties of Essential Oils and Their Compounds for Application in Skin Fungal Infections: Conventional and Nonconventional Approaches. Molecules, 2021 Feb 19; 26(4):1093. doi: 10.3390/molecules26041093.
Nazzaro, F., Fratianni, F., Coppola, R., & Feo, V. Essential Oils and Antifungal Activity. Pharmaceuticals (Basel), 2017 Nov 2; 10(4):86. doi: 10.3390/ph10040086.
Abdi-Moghadam, Z., Mazaheri, Y., Rezagholizade-Shirvan, A., Mahmoudzadeh, M., Sarafraz, M., Mohtashami, M., et al. The Significance of Essential Oils and Their Antifungal Properties in the Food Industry: A Systematic Review. Heliyon, 2023 Oct 29; 9(11):e21386. doi: 10.1016/j.heliyon.2023.e21386.
Tian, F., Woo, S. Y., Lee, S. Y., Park, S. B., Zheng, Y., & Chun, H. S. Antifungal Activity of Essential Oil and Plant-Derived Natural Compounds Against Aspergillus flavus. Antibiotics (Basel), 2022 Dec 1; 11(12):1727. doi: 10.3390/antibiotics11121727.
Noore, S., Rastogi, N. K., O’Donnell, C., & Tiwari, B. Novel Bioactive Extraction and Nano-Encapsulation. Encyclopedia, 2021, 1, 632-664. https://doi.org/10.3390/encyclopedia1030052.
Grandini, A., Summa, D., Costa, S., Buzzi, R., Tamburini, E., Sacchetti, G., & Guerrini, A. Biotransformation of Waste Bile Acids: A New Possible Sustainable Approach to Anti-Fungal Molecules for Crop Plant Bioprotection? International Journal of Molecular Sciences, 2022; 23(8):4152. https://doi.org/10.3390/ijms23084152.
Elhamouly, N. A., Hewedy, O. A., Zaitoon, A., Miraples, A., Elshorbagy, O. T., Hussien, S., et al. The Hidden Power of Secondary Metabolites in Plant-Fungi Interactions and Sustainable Phytoremediation. Frontiers in Plant Science, 2022; 13 https://doi.org/10.3389/fpls.2022.1044896.
Jeandet, P., Hébrard, C., Deville, M. A., Cordelier, S., Dorey, S., Aziz, A., & Crouzet, J. Deciphering the Role of Phytoalexins in Plant-Microorganism Interactions and Human Health. Molecules, 2014 Nov 5; 19(11):18033-56. doi: 10.3390/molecules191118033.
Borges, A., Abreu, A. C., Dias, C., Saavedra, M. J., Borges, F., & Simões, M. (2016). New Perspectives on the Use of Phytochemicals as an Emergent Strategy to Control Bacterial Infections Including Biofilms. Molecules, 21(7), 877. https://doi.org/10.3390/molecules21070877
Sobolev, V., Arias, R., Goodman, K., Walk, T., Orner, V., Faustinelli, P., & Massa, A. Suppression of aflatoxin production in Aspergillus species by selected peanut (Arachis hypogaea) stilbenoids. Journal of Agricultural and Food Chemistry, 2018; 66(1): 118–126.
da Silva, J. K., Silva, J. R., Nascimento, S. B., da Luz, S. F., Meireles, E. N., Alves, C. N., Ramos, A. R., & Maia, J. G. Antifungal activity and computational study of constituents from Piper divaricatum essential oil against Fusarium infection in black pepper. Molecules, 2014; 19(11): 17926–17942.
Gwad, M. M. A., El-Sayed, A. S. A., Abdel-Fattah, G. M., et al. Potential fungicidal and antiaflatoxigenic effects of cinnamon essential oils on Aspergillus flavus inhabiting the stored wheat grains. BMC Plant Biology, 2024; 24: 394.
Abd El-Aziz, A. R., Mahmoud, M. A., Al-Othman, M. R., Al-Gahtani, M. F. Use of selected essential oils to control aflatoxin contaminated stored cashew and detection of aflatoxin biosynthesis gene. Scientific World Journal, 2015; 2015: 958192.
Ejike, C. E. C., Gong, M., & Udenigwe, C. (2013). Phytoalexins from the Poaceae: Biosynthesis, function and prospects in food preservation. Food Research International, 52, 167-177. https://doi.org/10.1016/J.Foodres.2013.03.012
Kaiser, N., Douches, D., Dhingra, A., Glenn, K. C., Herzig, P. R., Stowe, E. C., & Swarup, S. (2020). The role of conventional plant breeding in ensuring safe levels of naturally occurring toxins in food crops. Trends in Food Science & Technology, 100, 51-66. https://doi.org/10.1016/j.tifs.2020.03.042
Ayaz, M., Li, C.-H., Ali, Q., Zhao, W., Chi, Y.-K., Shafiq, M., Ali, F., Yu, X.-Y., Yu, Q., Zhao, J.-T., Yu, J.-W., Qi, R.-D., & Huang, W.-K. (2023). Bacterial and Fungal Biocontrol Agents for Plant Disease Protection: Journey from Lab to Field, Current Status, Challenges, and Global Perspectives. Molecules, 28(18), 6735. https://doi.org/10.3390/molecules28186735
Poroșnicu, I., Ailincăi, L.-I., Neculai-Văleanu, A.-S., Ariton, A.-M., Mareș, M. (2024). Effects of Mycotoxins on the Health Status of Dairy Cattle. Scientific Papers: Animal Science and Biotechnologies, Vol. 57, No. 2, pp. 69-78.
Ałtyn, I., & Twarużek, M. (2020). Mycotoxin Contamination Concerns of Herbs and Medicinal Plants. Toxins, 12(3), 182. https://doi.org/10.3390/toxins12030182
Makhuvele, R., Naidu, K., Gbashi, S., Thipe, V. C., Adebo, O. A., Njobeh, P. B. (2020). The use of plant extracts and their phytochemicals for control of toxigenic fungi and mycotoxins. Heliyon, 6(10), e05291. https://doi.org/10.1016/j.heliyon.2020.e05291
Quesada-Vázquez, S., Codina Moreno, R., Della Badia, A., Castro, O., & Riahi, I. (2024). Promising Phytogenic Feed Additives Used as Anti-Mycotoxin Solutions in Animal Nutrition. Toxins, 16(10), 434. https://doi.org/10.3390/toxins16100434
More, P., Jambrak, A. R., & Arya, S. S. (2022). Green, environment-friendly and sustainable techniques for extraction of food bioactive compounds and waste valorization. Trends in Food Science & Technology, 128, October 2017. https://doi.org/10.1016/j.tifs.2022.08.016
Nieto, G., Martínez-Zamora, L., Peñalver, R., Marín-Iniesta, F., Taboada-Rodríguez, A., López-Gómez, A., & Martínez-Hernández, G. B. (2024). Applications of Plant Bioactive Compounds as Replacers of Synthetic Additives in the Food Industry. Foods, 13(1), 47. https://doi.org/10.3390/foods13010047
Das, S., Ghosh, A., Mukherjee, A. (2021). Nanoencapsulation-Based Edible Coating of Essential Oils as a Novel Green Strategy Against Fungal Spoilage, Mycotoxin Contamination, and Quality Deterioration of Stored Fruits: An Overview. Frontiers in Microbiology, 12, 768414. https://doi.org/10.3389/fmicb.2021.768414
Kumar, R., Kumar, N., Rajput, V. D., Mandzhieva, S., Minkina, T., Saharan, B. S., Kumar, D., Sadh, P. K., Duhan, J. S. (2022). Advances in Biopolymeric Nanopesticides: A New Eco-Friendly/Eco-Protective Perspective in Precision Agriculture. Nanomaterials (Basel), 12(22), 3964. https://doi.org/10.3390/nano12223964
Souto, A. L., Sylvestre, M., Tölke, E. D., Tavares, J. F., Barbosa-Filho, J. M., & Cebrián-Torrejón, G. (2021). Plant-Derived Pesticides as an Alternative to Pest Management and Sustainable Agricultural Production: Prospects, Applications and Challenges. Molecules, 26(16), 4835. https://doi.org/10.3390/molecules26164835
Ayilara, M. S., Adeleke, B. S., Akinola, S. A., Fayose, C. A., Adeyemi, U. T., Gbadegesin, L. A., Omole, R. K., Johnson, R. M., Uthman, Q. O., Babalola, O. O. (2023). Biopesticides as a promising alternative to synthetic pesticides: A case for microbial pesticides, phytopesticides, and nanobiopesticides. Frontiers in Microbiology, 14, 1040901. https://doi.org/10.3389/fmicb.2023.1040901
Nerilo, S. B., Romoli, J. C. Z., Nakasugi, L. P., Zampieri, N. S., Mossini, S. A. G., Rocha, G. H. O., Micotti da, E. G., Abreu Filho, B. A., & Machinski, M. Jr. Antifungal Activity and Inhibition of Aflatoxins Production by Zingiber officinale Roscoe Essential Oil Against Aspergillus flavus in Stored Maize Grains. Ciência Rural, 2020; 50(6):11.
Adekoya, I., Njobeh, P., Obadina, A., Landschoot, S., Audenaert, K., Okoth, S., De Boevre, M., & De Saeger, S. Investigation of the Metabolic Profile and Toxigenic Variability of Fungal Species Occurring in Fermented Foods and Beverages from Nigeria and South Africa Using UPLC-MS/MS. Toxins (Basel), 2019; 11(2):85. doi: 10.3390/toxins11020085.
Ponzilacqua, B., Rottinghaus, G. E., Landers, B. R., & Oliveira, C. F. Effects of Medicinal Herb and Brazilian Traditional Plant Extracts on In Vitro Mycotoxin Decontamination. Food Control, 2019; 100:24–2