In vitro and in situ Antibacterial and Insecticidal Activity of Fragaria ananassa Essential Oil
Keywords:
insecticidal activity, antibacterial potential in vitro and in situ, Fragaria ananassa essential oilAbstract
In folk medicine, the fruits and leaves of Fragaria ananassa have been used as a source of vitamins, as an agent in the treatment of inflammation, and as a diuretic, diarrhea and choleretic agent. It is used in the treatment of anaemia, in diseases of the gastrointestinal tract. Besides, the leaves have a styptic, astringent, wound-healing effect and are used for preparation of infusions, tinctures and tea. Infusion and decoction of leaves are applied at inflammation of mucous membranes of an oral cavity, at a headache, at jaundice, as an antiseptic and externally - at rashes and dermatitis. Infusion of leaves of F. ananassa shows antibacterial activity with respect to various microorganisms. The essential oil of F. ananassa was used in this study to determine its antibacterial potential against Gram-negative and Gram-positive bacteria and its insecticidal activity against insect. Bacteria from the Czech Collection of Microorganisms Bacillus subtilis, Paenibacillus larvae and Listeria ivanovii from the group of Gram-positive bacteria and Enterobacter aerogenes, Klebsiella pneumoniae and Shigella sonnei from the group of Gram-positive bacteria were used in the study to test the antibacterial activity. Megabruchidius dorsalis was used as the model insect for insecticidal activity. The best antimicrobial activity was found against P. larvae under in vitro conditions, as well as under in situ conditions on pear and parsley models. Insecticidal activity against M. dorsalis was observed at the highest concentrations. F. ananassa essential oil demonstrated very good antibacterial activity against various bacterial species under in vitro and in situ conditions, as well as good potential against insects.
References
Maurya, A., Prasad, J., Das, S., Dwivedy, A.K., Essential Oils and Their Application in Food Safety. Front. Sustain, Food Systems, 2021, 5, 133.
Amorati, R., Foti, M.C., Valgimigli, L., Antioxidant activity of essential oils, Journal of Agriculture and Food Chemistry, 2013, pp. 10835–10847.
Sharma, S., Barkauskaite, S., Jaiswal, A.K., Jaiswal, S., Essential oils as additives in active food packaging, Food Chemistry, 2021, pp. 128403. DOI:10.1016/j.foodchem.2020.128403.
Do Nascimento, L.D., de Moraes, A.A.B., da Costa, K.S., Galúcio, J.M.P., Taube, P.S., Costa, C.M.L., Cruz, J.N., de Aguiar Andrade, E.H., de Faria, L.J.G., Bioactive natural compounds and antioxidant activity of essential oils from spice plants: New findings and potential applications, Biomolecules 2020, pp. 988, DOI: doi.org/10.3390/biom10070988.
Vianna, T.C., Marinho, C.O., Marangoni Júnior, L., Ibrahim, S.A., Vieira, R.P., Essential oils as additives in active starch-based food packaging films: A review, International Journal of Biological Macromolecules, 2021, pp. 1803–1819. DOI: 10.1016/j.ijbiomac.2021.05.170
del Carmen Razola-Díaz, M., Guerra-Hernández, E.J., García-Villanova, B., Verardo, V., Recent developments in extraction and encapsulation techniques of orange essential oil, Food Chemistry, 2021, pp. 129575. DOI: 10.1016/j.foodchem.2021.129575
Kačániová, M., Čmiková, N., Vukovic, N. L., Verešová, A., Bianchi, A., Garzoli, S., Ben Saad, R., Ben Hsouna, A., Ban, Z., Vukic, M. D., Citrus limon Essential Oil: Chemical Composition and Selected Biological Properties Focusing on the Antimicrobial (In Vitro, In Situ), Antibiofilm, Insecticidal Activity and Preservative Effect against Salmonella enterica Inoculated in Carrot, Plants, 2004, 524. DOI: 10.3390/plants13040524
Karanth, S., Feng, S., Patra, D., Pradhan, A.K., Linking microbial contamination to food spoilage and food waste: The role of smart packaging, spoilage risk assessments, and date labeling, Frontiers in Microbiology, 2023, DOI:10.3389/fmicb.2023.1198124.
Damotharan, G. Sudhakaran, M. Ramu, M. Krishnan, K.R.N., Arockiaraj S.J., Biochemical processes mediating neurotoxicity induced by synthetic food dyes: A review of current evidence, Chemosphere, 2024, 143295,
DOI:10.1016/j.chemosphere.2024.143295.
Subbarayudu, S., Namasivayam, S.K.R., Arockiaraj, J., Immunomodulation in Non-traditional Therapies for Methicillin-resistant Staphylococcus aureus (MRSA) Management, Current Microbiology, 2024, pp. 346, DOI:10.1007/s00284-024-03875-7
Lawrence, H. A., Activity of Essential Oils Against Bacillus subtilis Spores, Journal of Microbiology and Biotechnology, 2009, pp. 1590–1595,
DOI:10.4014/jmb.0904.04016
Messager, S., Hammer, K.A., Carson, C.F., Riley, T.V., Sporicidal activity of tea tree oil, Australian Infection Control, 2006, pp. 112-121.
Pedrós-Garrido, S., Clemente, I., Calanche, J. B., Condón-Abanto, S., Beltrán, J. A., Lyng, J. G., Brunton, N., Bolton, D., Whyte, P., Antimicrobial activity of natural compounds against Listeria spp. and their effects on sensory attributes in salmon (Salmo salar) and cod (Gadus morhua), Food Control, 2020, 106768. DOI:10.1016/j.foodcont.2019.106768
Santos, R. C. V., Alves, C. F. dos S., Schneider, T., Lopes, L. Q. S., Aurich, C., Giongo, J. L., Brandelli, A., de Almeida Vaucher, R., Antimicrobial activity of Amazonian oils against Paenibacillus species, Journal of Invertebrate Pathology, 2012, 265–268, DOI:10.1016/j.jip.2011.12.002
Gutierrez, J., Rodriguez, G., Barry-Ryan, C., & Bourke, P., Efficacy of Plant Essential Oils against Foodborne Pathogens and Spoilage Bacteria Associated with Ready-to-Eat Vegetables: Antimicrobial and Sensory Screening, Journal of Food Protection, 2008, 1846–1854, DOI:10.4315/0362-028x-71.9.1846
Lambert, R. J. W., Skandamis, P.N., Coote, P., Nychas. G.J.E., A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol, Journal of Applied Microbiology, 2001, p. 453–462, DOI: 10.1046/j.1365-2672.2001.01428.x.
Oussalah, M., Caillet, S., Saucier, L., Lacroix, M., Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E. coli O157:H7, Salmonella Typhimurium, Staphylococcus aureus and Listeria monocytogenes, Food Control, 2006, pp. 414–420, DOI: 10.1016/j.foodcont.2005.11.009.
