Physico-Chemical and Mycological Evaluation of Fodder from a Cattle Farm


  • Ioana Poroșnicu Iasi University of Life Sciences, 700490, Mihail Sadoveanu Alley, no.3, Romania
  • Luminiţa-Iuliana Ailincăi Iasi University of Life Sciences, 700490, Mihail Sadoveanu Alley, no.3, Romania
  • Andra-Sabina Neculai-Văleanu Research and Development Station for Cattle Breeding, 707252, Iasi-Ungheni no.9, Dancu, Iasi
  • Mirela-Adina Ariton Research and Development Station for Cattle Breeding, 707252, Iasi-Ungheni no.9, Dancu, Iasi
  • Mihai Mareș Iasi University of Life Sciences, 700490, Mihail Sadoveanu Alley, no.3, Romania


contamination, fodder, mycological analyses, physical-chemical analyses


The purpose of the research was to carry out some analyses in order to establish the physico-chemical composition and the fungal load of the plant substrates used for raising animals, in a cattle farm in the Moldova area in 2022. There were five categories of samples (75 determinations in total) - alfalfa hay, corn silage, mixed feed ration, concentrates, corn grains and were analysed randomly from the farm. The results of the physico-chemical composition of the feed by the FT-NIRS technique were in accordance with the regulations in force and no significant differences were found between the samples, it is certain that the humidity had a higher percentage, hence the fact that there were developed species of fungi speaking from the point of view of mycotic contamination. The highest fungal load was recorded in corn grains and corn silage, and the lowest value was recorded in alfalfa hay. The number of colony-forming units per gram of sample was determined by the serial dilution technique in a double agar layer. The results obtained during the analysed period highlighted the presence of the genus Penicillium to the greatest extent (28%), and the lowest percentage was recorded for the genus Cladosporium (5.3%).


Santos, P.C., Cunha, S., amd Fernandes, J. O., Prevalent Mycotoxins in Animal Feed: Occurrence and Analytical Methods. Toxins, 2019, 11(5), 290. doi:10.3390/toxins11050290

Oldenburg, E., Höppner, F., Ellner F., Weinert, J., Fusarium diseases of maize associated with mycotoxin contamination of agricultural products intended to be used for food and feed. Mycotoxin Research, 2017, Vol. 33 (3), p. 167–182.

Alonso, V. A., Pereyra, C. M., Keller, L. A. M., Dalcero, A. M., Rosa, C. A. R., Chiacchiera, S. M., and Cavaglieri, L. R., Fungi and mycotoxins in silage: an overview. Journal of Applied Microbiology, 2013, 115(3), 637–643. doi:10.1111/jam.12178

Alemayehu, T., Yayneshet, T., Comparative chemical composition evaluation of local brewery and liquor byproducts made from different ingredients. Journal of the Drylands. 2011;4(2):307–309.

Amigot, S.L., Fulgueira, C.L., Bottai, H. and Basılico, J.C., New parameters to evaluate forage quality. Postharvest Biol Tec, 2006, 41, 215–224.

Gallo, A., Bertuzzi. T., Giuberti. G., Moschini, M., Bruschi, S., Cerioli, C., Masoero, F., New assessment based on the use of principal factor analysis to investigate corn silage quality from nutritional traits, fermentation end products and mycotoxins. J. Sci. Food Agric. 2016; 96:437–448. doi: 10.1002/jsfa.7109

Chen, L., Yang, Z., and Han, L., A Review on the Use of Near-Infrared Spectroscopy for Analyzing Feed Protein Materials. Applied Spectroscopy Reviews, 2013, 48(7), 509–522. doi:10.1080/05704928.2012.756403

Kung, L., Shaver, R. D., Grant, R. J., and Schmidt, R. J., Silage review: Interpretation of chemical, microbial, and organoleptic components of silages. Journal of Dairy Science, 2018, 101(5), 4020–4033. doi:10.3168/jds.2017-13909

Porosnicu I., Ailincai L.I., Neculai-Valeanu A.S., Madescu B.M., Ariton M.A., Crivei I., Mares M., Study on the fungal contamination of silage fodder and feed additives, Medicină Veterinară, 20-21 October 2022, Iași

Porosnicu I., Ariton A.M., Bors S.I., Badilas N.I., Coman I., Mycological investigations regarding the dominant flora from the vegetal substrates harvested from N-E Romania, Lucrări științifice, Seria Horticultură 64 (1/2), 2021, USV Iași

Swart, E., Brand, T., and Engelbrecht, J., The use of near infrared spectroscopy (NIRS) to predict the chemical composition of feed samples used in ostrich total mixed rations. South African Journal of Animal Science, 2012, 42(5). doi:10.4314/sajas.v42i5.22

Evangelista, C., Basiricò, L., and Bernabucci, U., An Overview on the Use of Near Infrared Spectroscopy (NIRS) on Farms for the Management of Dairy Cows. Agriculture, 2021, 11(4), 296. doi:10.3390/agriculture11040296

SR EN ISO 6887-1:2017 - Microbiology of the food chain - Preparation of test samples for microbiological examination - General rules the preparation of initial suspension and decimal dilutions.






Technologies Applied in Animal Husbandry