Association of SNPs in Porcine Estrogen Receptor Gene with Carcass Traits
Keywords:
associations’ analysis, porcine ESR gene, polymorphism, production traitAbstract
The aim of this study was to assess the influence of estrogen receptor gene polymorphism on carcass traits in pigs’ population. In association analysis was evaluated the effect of ESR/PvuII genotypes on back fat thickness, proportion of valuable meat parts, area of musculus longisimus thoracis (MLT) and proportion of thigh. In total 180 genomic DNA samples of crossbreeds (Large White x Landrace) was genotyped by using PCR-RFLP method and restriction enzyme PvuII. In population was observed prevalence of heterozygous individuals (45.56%). The lowest proportion was detected for homozygous BB genotype (13.89%). Genotype frequencies in analysed population caused the higher frequency of A allele (0.63±0.03) and relative high level of observed heterozygosity (0.46). The medium level of polymorphic information content (0.36) was found for selected locus. Association analysis was carried out by One-Way ANOVA procedure. The results showed that genotype of ESR gene influenced significantly only proportion of thigh and area of MLT (P<0.01). Average values of both traits observed for each genotype indicated positive effect of A allele present in genotype. Our results demonstrate the significant role of ESR gene for economically important traits that can be used for application in marker assisted selection programs of pigs.
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