Effects of Semen Sexing Agent (Heifer-PlusTM) in Dairy Cow Reproduction


  • Constantin Găvan Research Department, Agriculture Research and Development Station Șimnic, Craiova, Romania
  • Mihaela Riza Research Department, Agriculture Research and Development Station Șimnic, Craiova, Romania


Heifer-Plus, artificial insemination, fetal sex, conception rate


This study was conducted to investigate the use cryopreserved bovine sperm treated with Heifer-PlusTM Prefreeze kit and its effect on pregnancy rates calf sex ratio embryonic death, abortion, stillbirth and twinning rates in Holstein Friesian cows A total of 120 Holstein Friesian cows were enrolled in this study. Sixty cows were artificially inseminated (AI) whit cryopreserved bovine sperm treated with Heifer-PlusTM PREFREEZE kit (experimental group; EG) and 60 cows served as control group (CG).

Findings showed that the AI of cows with bovine semen treated with Heifer PlusTM Prefreeze kit had slight increases in conception rate (5%) and in female and male ratio (18.5%). Use of cryopreserved bovine sperm treated with Heifer-PlusTM Prefreeze kit did not affected embryonic death, abortion, stillbirth and twinning ratios in Holstein Friesian cows. Further investigations are needed with larger numbers of tested dairy cows, which enable wide spread adoption of this technology


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