STABILIZATION OF MICROBIAL ENZYMATIC PREPARATIONS USED IN FEED INDUSTRY

Authors

  • MONICA DRAGOMIRESCU Faculty o f Animal Sciences and Biotechnologies, Timişoara, România
  • T. VINTILĂ Faculty o f Animal Sciences and Biotechnologies, Timişoara, România
  • BEATRICE VLAD-OROS Faculty o f Chemistry-Biology-Geography, West University o f Timişoara, România
  • GABRIELA PREDA Faculty o f Chemistry-Biology-Geography, West University o f Timişoara, România

Keywords:

feed enzymes, microbal proteases, Bacillus licheniformis, immobilization, sol-gel, TEOS

Abstract

The enzymatic compound with protease activity obtained by Bacillus licheniformis
CM1T 1.33 cells fermentation was entrapped in sol-gel matrixes, using TEOS as
precursor. The sol-gel technique is a soft method for silica glass synthesis at room
temperature. In silica gels, the enzymes maintain their catalytic activity for a longer
period o f time and present high stability at environmental factors. Bacillus
licheniformis CMIT 1.33 enzyme was also immobilized by entrapment in silica gels
deposited on a ceramic support. The optimal temperature and pH o f the native and.
immobilized enzyme did not vary significantly. At temperature and pH values lower
than the optimum, the relative activities have been higher for the immobilized
compound. The immobilization o f the enzymatic compound increased the stability.
The enzymatic compounds with protease activity obtained by silica gels entrapment
and entrapment/deposition on ceramic support can be used as forage additive.

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Published

2023-11-01