COMPUTER-ASSISTED SEMEN ANALYSIS OF RAT SPERMATOZOA AFTER AN INTRAPERITONEAL ADMINISTRATION OF INSECTICIDE DIAZINON

Authors

  • R. TOMAN Slovak Agricultural University in Nitra, Slovak Republic
  • S. HLUCHÝ Slovak Agricultural University in Nitra, Slovak Republic
  • P. MASSÁNYI Slovak Agricultural University in Nitra, Slovak Republic
  • B. ŠIŠKA Slovak Agricultural University in Nitra, Slovak Republic
  • N. LUKÁČ Slovak Agricultural University in Nitra, Slovak Republic
  • J. SLIVKOVÁ Slovak Agricultural University in Nitra, Slovak Republic
  • J. GOLIAN Slovak Agricultural University in Nitra, Slovak Republic
  • P. ČUPKA Slovak Agricultural University in Nitra, Slovak Republic

Keywords:

diazinon, CASA, rat, spermatozoa, motility

Abstract

The aim of this study was to reveal the effect of diazinon on the rat spermatozoa
motility characteristics using the computer-assisted semen analysis (CASA).
Motility, progressive motility, DAP, DCL, DSL, VAP, VCL, VSL, STR, LIN, WOB,
ALH, and BCF after the diazinon i.p. administration of 20 mg/kg b.w. were
evaluated. 36 hours after the diazinon administration, only slight decrease in VCL,
DCL and increase in percentage of progressive motility in the diazinon-treated
group. Significant decrease (P<0.01) was only observed in BCF in diazinon-treated
group. Computer-assisted semen analysis (CASA) of rat sperm motility showed that
acute diazinon administration slightly affected the rat sperm motility which can be
the first step in the decreased fertilization capacity caused by pesticides. Further
investigation of reproductive effects of diazinon is needed.

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Published

2023-11-01