Control on the Wheat Non-Starch Polysaccharides (NSP)’ Anti-Nutritional Effect on Intestinal Wall, by Introducing Xylanase in Broiler Feed
Keywords:
broiler, intestinal wall, non-starch polysaccharides, xylanase, wheatAbstract
The experiment was performed in order to determine the protocol of xylanase utilization to fight against the anti-nutritional effect exerted by wheat non-starch polysaccharides (NSP) on intestinal wall in broilers. The experimental works were carried out on broilers, the hybrid Ross 308. We formed four experimental groups, as follows: the experimental group LE1, fed on forage without wheat in its structure, the experimental group LE2, fed on combined forage including wheat in a proportion of 40%, the experimental group LE3 including wheat in a proportion of 40% and xylanase, in an amount of 25 g / to, and the experimental group LE4, including wheat in a proportion of 40% and xylanase, in an amount of 100 g. At 3 and 6 weeks, successive to chicken killing, we sampled the intestinal wall and determined the main changes occurred. The histo-morpho-metric analysis of the four experimental groups led to the conclusions that: wheat administration in a proportion of 40% in the individuals in LE2 determines the development of vilositary muscle elements, decreased of intestinal villosities height, leucocitary migration, and also vascular ectasies and reduced hemorrhagic areas; xylanase addition in the wheat-based feed may be associated with the increase of intestinal villosities height, and especially at jejuna level the villosities seem slightly branched, a slight hypertrophy of the epithelial cells compared with the individuals in LE2, the increase of goblet cells frequency and hypertrophy of the capillary network. These microscopic aspects come together with more intens digestion and absorption processes, and especially in the experimental group 4.
References
Austin, S., Wiseman, C., Chesson, J., Influence of non starch polysaccharides structure on the metabolisable energy of U. K. Wheat fed poultry, 1999.
Steenfeldt, S., Mulllertz, A., Fries Jensen, J., The dietary effect of different wheat cultivars for broiler chickens. Br. Poultry Sci., 1998, 42, 595-609
Cummings, J. H., Short chain fatty acids in the human colon, J Br Soc Gastroent, 1981, 22, 763-779
***Ross Breeder Management Guide, Management guide for all Ross parent stock Breeder the art of genetic science, www.aviagen.com/pdf/ Ross _NA/US%20 Ross %20 Breeder%20manual.htm .NRC (1994) Nutrient Requirements of Poultry, 9th edn. National Academy of Sciences, Washington, DC
Simon, O., The mode of action of NSP hydrolysing enzymes in the gastrointestinal tract, Journal of Animal Feed Sciences, 2005, 7, 115-123
Dusel, G., Kluge, H., Jeroch, H., Xylanase suplementation of wheat based rations for broilers influence of wheat characteristics, J. Appl. Poultry Res., 1998, 7, 119-131
Englyst, H., Classification and measurement of plant polysaccharides, Anim. Feed Sci. Technol., 1989, 23, 27-42
Irish, G. G., Balnave, D., Non starch polysaccarides and broiler performance on diets containing sozabean meal as the sole protein concentrate, Aaust J. Agric Res., 1993, 8, 381-390
McDonald, D. E., Dietary fibre for the newly weaned pig: Influences on pig performance, intestinal development and expression of experimental postweaning colibacillosis and intestinal spirochaetosis. PhD Thesis, Murdoch University, Murdoch, 2001.
Iji, P. A., Saki, A. A., & Tivey, D. R., Intestinal development and body growth of broiler chicks on diets supplemented with non-starch polysaccharides. Animal Feed Science and Technology, 2001, 89, 175–188
Leenhouwers, J. I., Adjei, B. D., Verreth, J. A. J., & Schrama, J. W., Digesta viscosity, nutrient digestibility and organ weights in African catfish (Clarias gariepinus) fed diets supplemented with different levels of a soluble non-starch polysaccharide, Aquaculture Nutrition, 2006, 12, 111–116
Jin, L., Modulation of feed intake by weanling pigs. Ph.D. Dissertation, North Dakota State University, Fargo, 1992.
Hopwood, D. E., Pethick, D. W., & Hampson, D. J., Increasing the viscosity of the intestinal contents stimulates proliferation of enterotoxigenic Escherichia coli and Brachyspira pilosicoli in weaner pigs, British Journal of Nutrition, 2002, 88, 523–532
Montagne, L., Pluske, J. R., & Hampson, D. J. A, Review of interactions between dietary fibre and the intestinal mucosa, and their consequences on digestive health in young non-ruminant animals, Animal Feed Science and Technology, 2003, 108, 95–117
Bedford, M. R., Classen, H. L., Reduction of intestinal viscosity through manipulation of dietary rye and pentosanase concentration is effected through changes in the carbohydrate composition of the intestinal aqueous phase and results in improved growth rate and food conversion efficiency of broiler chicks, J. Nutr., 1992, 122, 560–569
Bedford, M. R., Exogenous enzymes in monogastric nutrition—their current value and future benefits, Anim. Feed Sci. Technol., 2000, 86, 1–13
Bedford, M.R., Schulze, H., Exogenous enzyme for pigs and poultry, Nutr. Res. Rev., 1998, 11, 91–114
Meng, X., Slominski, B. A., Nyachoti, C. M., Campbell, L. D., Guenter, W.,. Degradation of cell wall polysaccharides by combinations of carbohydrase enzymes and their effect on nutrient utilization and broiler chicken performance, Poult. Sci., 2005, 84, 37–47
Viveros, Aa., Brenes, A., Pizzaro, M., and Castano, M., Effect of enzyme supplementation of a diet based on barley, an autoclave treatment, on apparent digetsibility, growth performance and gut morphology of broilers, Animal Feed Science and Technology 1994, 48, 237-251
Engberg, R. M., Hedemann, M. S., Steenfeldt, S., Jensen, B. B., Influence of whole wheat and xylanase on broiler performance and microbial composition and activity in the digestive tract, Poult. Sci., 2004, 83, 925–938
Choct, M., Hughes, R. J., Bedford, M. R., Effects of a xylanase on individual bird variation, starch digestion throughout the intestine, and ileal and caecal volatile fatty acid production in chickens fed wheat, Br. Poult. Sci., 1999, 40, 419–422.
