Quality Criteria for Propolis Standardization


  • Laura Stan University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca - 400372 Cluj-Napoca, Calea Manastur 3-5, Romania
  • Liviu Al. Mărghitaș University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca - 400372 Cluj-Napoca, Calea Manastur 3-5, Romania
  • Daniel Dezmirean University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca - 400372 Cluj-Napoca, Calea Manastur 3-5, Romania


antioxidant activity, DPPH, flavonoids, quality standard of propolis, phenolic compounds, propolis


Propolis is a natural resine produced by honeybees with high biological value for human kind. Although propolis is intensively used in medicine, cosmetics and lately in food industry also, there is no quality standard (Romanian or European) for this specific beeproduct. This article presents the quality criteria for Romanian raw propolis (56 samples) as a collection of data obtained along the years through extensive qualitative and quantitative analysis. Data obtained for extraction yield (59%±8%), wax content (35%±8%), total flavonoids (9£±3%) and total phenolics (28%±7%), antioxidant activity against DPPH are presented.


Bankova, V., Chemical diversity of propolis and the problem of standardization, J. Ethnopharm., 2005, 100, 114–117

Bankova, V., Popova M, Trusheva B, Plant sources of Propolis: an update from a chemist’s point of view, Natural Product Communications, 2006, 0, 1-6

Mărghitaş, L. Al., Albinele şi produsele lor, Ed. Ceres, 2005

Sforcin, J. M., Bankova, V., Propolis: Is there a potential for development of new drugs?, J. Ethnopharmacol., 2010, doi: 10.1016/j.jep.2010.10.032

Burdock, GA, Review of the biological properties and toxicity of bee propolis (propolis), Food and Chemical Toxicology 1998, 36, 347-363

Mărghitaş, L. Al., Dezmirean, D., Moise, A., Mihai C. M., Stan, L, DPPH method for evaluation of propolis antioxidant activity, Bulletin USAMV, 2009, 66, 253-258

Ahn, M. R., Kumazawa, S., Usui, Y., Nakamura, J., Matsuka, M., Zhu, F., Nakayama, T., Antioxidant activity and constituents of propolis collected in various areas of China, Food Chemistry 2007, 101, 1383-1392

Kumazawa, S., Ahn, M. R., Fujimoto, T., Kato, M., Radical scavenging activity and phenolic constituents of propolis from different regions of Argentina, Natural Product Research 2010, 24/9, 804-812

Instituto Argentino de Normalization, Esquema 1 De Norma IRAM-INTA 15935-1, Productos del Noroeste Argentino: Propoleos, 2004

Lihong, C., Fuxing, Z., Xin, Z., Jianme, W., New quality standard of propolis in China, 10th AAA international Conference, 2010, 165

Huang, C. Y., Chen, Y. W., Chen, C. N., Standardization and characterization of Taiwanese green propolis, 10th AAA international Conference 2010, pp. 166

Lee, S. W., WPSF activities and proposal for international standardization of propolis, 10th AAA international Conference 2010, pp. 167

Popova, M., Bankova, V., Butovska, D., Petkov, V., Damyanova, B., Sabatini, A. G., Marcazzan, G. L., Bogdanov, S., Validated methods for the quantification of biologically active constituents of poplar type propolis, Phytochemical analysis 2004, 15, 235-240

Mihai, C. M., Mărghitaş, L. Al., Dezmirean, D., Maghear, O., Mărgăoan, R., Stan, L., Transylvanian propolis from 7 counties – qualitative and quantitative analysis of phenolics, Bulletin USAMV-CN, 2009, 66(1-2), 259-264

Mărghitaş, L. Al., Dezmirean, D., Laslo, L., Moise, A., Popescu, O., Maghear, O., Validated method for estimation of total flavonoids in Romanian propolis, Bulletin USAMV-CN, 2007, 63-64, 164-169

Mărghitaş, L. Al., Laslo, L, Dezmirean, D., Moise, A., Maghear, O., Total phenolics and antioxidant activity of Romanian propolis, International Conference Agricultural and Food Sciences, Progresses and Technologies, Sibiu, 2007, 209-212

Bankova, V. S., Determining quality in propolis samples, The Journal of the American Apitherapy Society, 2000, 7, 2-4

Silva, J. F. M., Souza, M. C., Matta, S. R., Andrade M. R., Vidal, F. V. N., Correlation analysis between phenolic levels of Brazilian propolis extracts and their antimicrobial and antioxidant activities, Food Chemistry, 2006, 99, 431-435






Fundamental Sciences in Animal Husbandry