Some Comparative Histomorphometrical Aspects Regarding Detoxifying Capacity of Garlic, Coriander and Chlorella, in Chronic Cd Contamination on Carassius gibelio Species
Keywords:
Cd intoxication, chlorella, coriander, freshwater fish, garlic, histomorphometric studyAbstract
The purpose of this study was to histomorphometrically compare the aspect of gill, intestinal and ovarian epithelium of Prussian carp specimens, simultaneously subjected to chronic intoxication with Cd and, to chelating and antioxidant effect of chlorella, coriander and garlic on this toxicant respectively.
150 Prussian carps, 10-12 g of weight were divided according to the following treatments for 21 days: C (without treatment), E1 (10 ppm Cd into water), E2 (10 ppm Cd into water+2% lyophilized garlic in feed), E3 (10 ppm Cd into water+2% lyophilized coriander in feed), E4 (10 ppm Cd into water+2% lyophilized chlorella in feed).
Fragments of gill, small intestine and ovary were removed and analyzed by light microscopy and a specific QuickPHOTO Micro 2.2 software has been used for the histomorphometric study.
Mentioned epithelium suffered evident histomorphologic and histomorphometric changes under the action of Cd and Cd plus chlorella, Cd plus coriander and Cd plus garlic respectively. Statistical processing data related to the gill lamellae length, intestinal villi height and chorion thickness of ovaries follicles revealed the existence of different degrees of significance between experimental groups compared.
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