GENETIC POLYMORPHISM OF SOME PROTEINS IN THE MILK OF CARPATHIAN GOAT
Keywords:
goats, milk polymorphism, caseinAbstract
The paper presents some aspects of the polymorphism of some Carpathian goat milk
proteins. The Carpathian breed is the main breed of goats reared in Romania. The
optimal working conditions were determined for the identification of the casein
phenotypes. The technique of the polyacrylamide gel electrophoresis was used. The
milk samples were processed to remove the fat and whey and the migration was
done in the presence of a standard sample which contained proteins with different
molecular weights. The interpretation of the electrophoresis migrations revealed the
presence of two genotypes, the homozygous genotype β-Cn BB and the heterozygous
β-Cn AB. The homozygous genotype β-Cn AA was not identified in any individual.
The heterozygous genotype β-Cn AB displayed a high frequency (59%) and it was
observed in 10 individuals. The homozygous genotype β-Cn BB was observed in 7
individuals and it had a frequency of 41%. The homozygous genotype β-Cn AA has
not been identified in the studied population. The distribution of these genotypes
showed that allele β-CnB was predominant (70%) over allele β-can (30%).
References
Maria Ballester, Armand Sanchez and Josep M., Folch (2005) –
Polymorphisms in the goat β-lactoglobulin gene, Jour. of Dairy Research, , 72 379-
S. Chessa, E. Budelli, K. Gutscher, A. Caroli and G. Erhardt (2003)–
Short comunication: simultaneous identification of five k-casein (CSN3) alleles in
domestic goat by polymerase chain reaction – Single strand conformation
polymorphism , J. Dairy Sci., 86:3726-3729
Christine Leroux, Fabienne Le Provost, Elisabeth Petit, Laurence
Bernard, Yves Chilliard, Patrice Martin (2004) – Real – Time RT-PCR and
cDNA macroarray to study the impact of the genetic polymorphism at the αs1 -
casein locus on the expression of genes in the goat mammary gland during
lactation , Reprod. Nutr. Dev. 43, 2003, 459-469, INRA, EDP Sciences
Ajay Kumar, Pramod Kumar Rout, Ramadhar Roy (2006)–
Polimorphism of β-lactoglobulin gene in Indian goats and its effect on milk yield ,
J. Appl Genet 47 (1)
Hiroyuki Tomotake, Ryoko Okuyama, Mitsuaki Katagiri and col
(2006).- Comparison between Holstain cow`s milk and Japanese – Saanen goat`s
milk in fatty acid composition, lipid digestibility and protein profile , Biosci.
Biotechnol. Biochem. 70 (11),(2771-2774)
M. H. Yahyaoui, A. Angiolillo, F. Pilla, A. Sanchez, and J. M. Folch
(2003)– Characterisation and genotyping of the caprine k – casein variants, J.
Dairy Sci., 86:2715-2720.
