RESEARCH CONCERNING THE INFLUENCE OF CAPACITATION CONDITIONS ON THE QUALITY OF SPERMATOZOA USED FOR IN VITRO FERTILIZATION
Keywords:
spermatozoa, capacitation, technique, mediaAbstract
The optimization of capacitation technique is based on the study of the influence
factors of this process. Studying the influence of capacitation conditions on boar
sperm quality we concentrated our research upon capacitation technique and
method. We used a dilution and washing method (centrifugation), a migration
method (swim-up) and a selective washing method (Percoll). The capacitation
medium acts and interacts with the capacitation technique and in order to study
these factors we used: Tyrode`s, TALP-HEPES and TCM 199 media. The results
showed that the capacitation technique influences the parameters of the sperm used
for embryo in vitro production and the technique depends on the quality of the raw
semen.
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