STUDY CONCERNING PRODUCTION OF CELLULASE ENZYMES IN SOLID STATE CULTURES OF TRICHODERMA VIRIDE
Keywords:
Cellulase, Trichoderma, SSF, LSF, cellulose hydrolysisAbstract
The hydrolysis of the lignocelluloses to fermentable sugars seems to be the main
reason for the high producing cost of ethanol from lignocelluloses. The objective
of this work is to test two strains of Trichoderma in liquid state and solid state
cultures for cellulase production, and to compare the productivity and efficiency
of the two systems of fermentation. Submerged liquid cultures (SLC) and solid
state cultures (SSC) were carried out to compare the productivity of the two
strains of Trichoderma. Comparing the productions of cellulases in the systems
applied in this study, data indicate the system of solid state culture with flushing as
the most efficient (660% more efficient in T. viride ATCC 13.631 SSC+f than in
SLC and 455% more efficient in T. viride CMGB1 SSC+f than in SLC). Still, T.
viride CMGB1 show a higher production (2160 FPU in SSC+f) than T. viride
ATCC 13.631 (1880 FPU in SSC+fm) in laboratory conditions. These results
recommend solid state cultures as systems for producing cellulases at lower price
than liquid state cultures. These low cost cellulases can lower the price of ethanol
produces from lignocellulosic biomass.
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