TESTING OF THE EFFICIENCY OF SOME ENZYMATIC MIXTURES, CONCERNING THE CONVERSION OF SEVERAL LIGNOCELLULOSIC BIOMASS’ SOURSES, TO REDUCING SUGARS
Keywords:
enzymatic mixtures, enzymatic hydrolysis, reducing sugars, efficiencyAbstract
Three lignocelluloses substrates have been used, as following: Mischantus, Maize
stalk and Wheat bran, in order to obtain fermentescible sugars, which will be
transformed into bioethanol. The substrates were hydrolysed using commercial
enzymes: MethaPlus (β-glucanase, xylanase, cellulase), Veron 191 (xylanase), Hep
C (cellulase). The hydrolysis was performed at 550 C, for 20 h, at pH = 5,5. The best
results were obtained by using MethaPlus enzyme. The efficiency of hydrolysis was
110.80 % for Miscanthus, 126.15 % for maize stalk and 118.76 % for wheat bran,
reported to the control. The most enhanced quantities of reducing sugars were
obtained in maize stalk, namely: 126.15 % using MethaPlus, 112.07 % using Veron
191 and 113.52 % using Hep C. The wheat bran was hydrolysed with enzymatic
mixtures, for emphasizing the reducing sugars’ grow, coming from residual starch’s
content (flour). In comparison to the control, the efficiency of hydrolysis was of:
181.004 % for MethaPlus-Veron M4 mixture, 168.83 % for MethaPlus-Veron 393
mixture, 205.86 % for MethaPlus-BG α-malt mixture and of 176.57 % for
MethaPlus-Veron MX mixture. The enzymatic mixture which contains BG α-malt
was the most productive, the hydrolysis efficiency being superior to all other
variants.
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EUROPEAN COMMISSION- EUR 21350 – BIOMASS, 2005 - Green
energy for Europe, Luxembourg: Office for Official Publications of the European
Communities.
