MOLECULAR CLONING OF OVINE cDNA LEPTIN GENE
Keywords:
ligation reaction, bacterial transformation, recombinants selectionAbstract
An efficient bacterial transformation system suitable for cloning the coding
sequence of the ovine leptin gene in E. coli DH5α host cells using the pGEMT easy
vector it is described in this paper. The necessity of producing leptin is based on the
fact that the role of this molecule in the animal and human organism is still
unknown, leptin not existing as commercial product on the Romanian market. The
results obtained in the bacterial transformation, cloning, recombinant clones
selection, control of the insertion experiments and DNA computational analysis
represent the first steps in further genetic engineering experiments such as
production of DNA libraries, DNA sequencing, protein expression, etc., for a further
contribution in elucidating the role of leptin in the animal and human organism.
References
Choe S.W., Nian R., Lai B.W. (2006) - Recent advances in bimolecular
process intensification ,Chemical Engineering Science vol.61 , 886-906
Hanchuan D., Liangqi L., Guang D. (2006) - Molecular cloning of the
obese gene from Cyprinus carpio and its expression in Escherichia coli, Front.
Biol. China 1, 50
Liang R., Liu X., Jianhua L., Ren Q., Liang P., Lin Z., Xie X., (2007) -
A T7-expresion system under temperature control could create temperature
sensitive phenotype of target gene in Escherichia coli, Journal of Microbiological ,
vol.68, 497
bio.org.
