DIAGNOSTICATION OF MALIGNANT HYPERTHERMIA IN SWINE
Keywords:
swine, malignant hyperthermia, PCR-RFLP, diagnosticationAbstract
Malignant hyperthermia (MH) causes neurological, liver, and kidney damage and
death in humans and major economic losses in the swine industry. A single point
mutation in the gene for the skeletal muscle ryanodine receptor (Ryrl) was found to
be correlated with MH in major breeds of swine. This mutation generates the
production of an abnormal protein which alters the structure and function of the
Ca2+ release channel of skeletal muscle sarcoplasmic reticulum (ryanodine
receptor). Because the mutation destroys the recognition site for the restriction
endonuclease HhaI, it was possible to develop a PCR-RFLP method for MH
diagnostication in all swine breeds. Our objective was to identify the normal
homozygous, heterozygous carriers and affected homozygous swine for MH trait,
using this method. Results suggest that the genetic test will be useful in identifying
heterozygous pigs for the MH trait.
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