Identification of the Bovine Growth Hormone Gene and Alui Loci Polymorphism by PCR – RFLP Method
Keywords:
Cattle, growth hormone gene, PCR-RFLP, polymorphismAbstract
The aim of this study was detection of polymorphism in the bovine growth hormone gene using PCR – RFLP as an efficient method for genetic analysis of polymorphism. A polymorphic site of the growth hormone gene (AluI polymorphism) that results in an amino acid change at position 127 of the protein chain (leucine, L to valine, V) has been linked to differences in circulating metabolites and metabolic hormones and to milk yield traits. The growth hormone gene is a candidate gene for body weight and weight gain in cattle since it plays a fundamental role in growth regulation. The polymorphism of growth hormone gene was studied in a group of 58 bulls of Slovak spotted breed. A strategy employing PCR was used to amplify 428 bp products from blood samples. Digestion of PCR products with restriction enzyme AluI revealed two alleles: allele L was 265, 96, 51, 16 fragments and allele V was 265, 147 and 16. Three patterns were observed and with frequencies 0.404, 0.473 and 0.123 for LL, LV and VV, respectively. The frequency of alleles L was 0.6404 and V was 0.3596.
References
Goodman, H. M., Growth hormone and metabolism. In: The endocrinology of growth, Development and Metabolism in vertebrates. M. P. Schreibman, C. G. Scanes and P. K. T. Pang, Ed. Academic Press, 1993, pp. 93-115
Etherton, T. D., Bauman, D. E., Biology of somatotropin in growth and lactation of domestic animals, Physiol. Rev., 1998, 78, 745-761
Svennersten-Sjaunja, K., Olsson, K., Endocrinology of milk production, Domest. Anim. Endocrinol., 2005, 29, 241-258
Burton, J. L., McBride, B. W., Block, E. and Glim, D. R., A review of bovine growth hormone, Can. J. Anim. Sci., 1994, 74, 167-201
Nicoll, C. S., Mayer, G. L. and Russell, S. M., Structural features of prolactin and growth hormones that can be related to their biological properties, Endocrinology Revue, 1986, 7, 169-203
Stasio, L. D., Destefanis, G., Brugiapaglia, A., Alberta, A. and Rolando, A., Polymorphism of the GHR gene in cattle and relationship with meat production and quality, Animal Genetics, 2005, 36, 138-140
Hediger, R., Johnson, S. E., Barendse, W., Drinkwater, R. D., Moore, S. S. and Hetzel, J., Assignment of the growth hormone gene locus to 19q26-qter in cattle and to 11q25-qter in sheep by in situ hybridization, Genomics, 1990, 8, 171-174
Ferraz, A. L., Bortolossi, J. C., Curi, R. A., Ferro, M. I. and Furlan, L. R. Identification and characterization of polymorphisms within the 5´ flanking region, first exon and part intron of bovine GH gene, Journal of Animal Breeding and Genetics, 2006, 123, 208-212
Silveira, L. G. G., Furlan, L. R., Curi, R. A., Ferraz, L. J., Alencar, M. M., Regitano, L. C. A., Martins, C. L., Arrigoni, M. B., Suguisawa, L., Silveira, A. C. and Oliveira, H. N., Growth hormone 1 gene (GH1) polymorphisms as possible markers of the production of beef cattle using the Brazilian Canchim breed as a model, Genetics and Molecular Biology, 2008, 31, 874-879
Lucy, M. C., Hauser, S. D., Eppard, P. J., Krivi, G. G., Clark, J. H., Bauman, D. E. and Colier, R. J., Variants of somatotropin in cattle: gene frequencies in major dairy breeds and associated milk production, Domest. Anim. Endocrinol., 1993, 10, 325-333
Lucy, M. C., Hauser, S. D., Eppard, P. J., Krivi, G. G. and Collier, R. J., Genetic polymorphism within the bovine somatotropin (bST) gene detected by polymerase chain reaction and endonuclease digestion, J. Dairy Sci., 1991, 74, 284
Lechniak, D., Machnik, G., Szydlowski, M. and Switonski, M., Growth hormone gene polymorphism and reproductive performance of bulls, Theriogenology, 1999, 52, 1145-1152
Balogh, O., Kovács, K., Kulcsár, M., Gáspárdy, A., Zsolnai, A., Kátai, L., Péci, A. Fésüs, L., Butler, W. R. and Huszenicza, G., AluI polymorphism of the bovine growth hormone (GH) gene, resumption of ovarian cyclicity, milk production and loss of body condition at the onset of lactation in dairy cattle, Theriogenology, 2009, 71, 553-559
Kovács, K., Völgyi-Csík, J., Zsolnai, A., Györkös, I. and Fésüs, L., Associations between the AluI polymorphism of growth hormone gene and production and reproduction traits in a Hungarian Holstein-Friesian bull dam population, Arch. Tierz., 2006, 49, 236-249
Jakaria, R., Noor, R., Martojo, H., Duryadi, D. and Tappa, B., Identification of growth hormone (Gh) Gene mspI and AluI Loci Polymorphism in Beef Cattles. Proc. The 1st International Seminar on Animal Industry, Bogor, Indonesia, 2009, pp. 42-46
