STUDIES REGARDING THE VIABILITY OF MOUSE EMBRYOS RECOVERED IN EARLY DEVELOPMENTAL STAGES
Keywords:
embryo, viability, culture medium, FAD, PIAbstract
In order to evaluate the embryo viability we tested 2 cultivation media in three
combinations (M16, Nutrient mixture F- 12 HAM supplemented with BSA and
Nutrient mixture F- 12 HAM without BSA). In the third day of cultivation the
embryos viability was assessed by morphological criteria and randomly by staining
with fluorescents dyes (Fluorescein diacetate and Propidium iodide). It was
observed that both media used for in vitro cultivation assured embryo development
up to the expanded blastocyst stage (21.33% for M 16 and 4.34% respectively),
exception the Nutrient mixture F- 12 HAM without BSA in which after 3 days
cultivation all embryos were degenerated. The M16 medium assured the embryos
development up to the hatched blastocyst stage (5.33%). None of the media
supported the development of embryos under 8 cell stages and early blockage in
stage of 2 cells could not be prevented.
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