CONSEQUENCES OF AD LIBITUM ALUMINIUM ADMINISTRATION DURING THREE GENERATIONS ON RATS BLOOD GSH DINAMICS
Keywords:
GSH, aluminium, ratsAbstract
The influence of ad libitum aluminium sulphate, Al2(SO4)3, administration during
three generations on the blood glutathione (GSH) values in rats was studied. This
study was carried out on 34 Wistar rats divided in 9 experimental batches (LI
, LII,
LIII, LIF1n, LIIF1n, LIIIF1n, LIF2n, LIIF2n, LIIIF2n )and one control batch (C).
Aluminium sulphate has been administrated in drinking water, ad libitum. GSH
was measured quantitatively at Perkin-Elmer spectrophotometer through Beutler
et. al. method (4), at 412nm. Hemoglobin (Hb) was measured through Drabkin
method (9) at the automatic analyzer Bekman Coulter. GSH is considered to be
the most powerful and most important of body’s self-generated antioxidants. It is a
powerful antioxidant, he neutralizes free radicals and prevents their formation.
Because aluminium (Al) can couse oxidative damage on cellular biological
processes by inhibiting GSH regeneration through the inhibition of NADPH
supply in mitochondria, but only a little inhibitory effect on the GSH generation in
cytosol (17), the consequences of ad libitum Al2(SO4)3 administration was a
limited decrease of GSH values.
References
Alfrey, A.C. (1980)- Aluminum metabolism in uremia, Neurotoxicology
:43-53
Alfrey, A.C. (1983)- Aluminum, Adv. Clin. Chem. 22:69-91.
Berlin, A. (1982-3)- Aluminum analysis in biological fluids, J. Toxicol.
din. Toxicol. 19:907-910
Beutler, E., Dubon, O. and Kelly B.M.( 1963) - J.Lab.Clin Med.
:882-890
Crapper McLachlan, D.R., Farnell, B.J. (1985)- Aluminum and
neuronal degeneration. In: Metal Ions in Neurology and Psychiatry. Ed. S. Gabay,
J. Harris, and B.T. Ho, Alan R. Liss, Inc., New York, 69-87.
Cronan, C.S., C.L., Schofield, C.L. (1979)- Aluminum leaching
response to acid precipitation: Effect on high-elevation watersheds in the
Northeast, Science 204:304-306.
Dunea, G. et al. (1978)- Role of aluminum in dialysis dementia, Ann.
Intern. Med. 88:502-504
Ganrot, P.O. (1986)- Metabolism and possible health effects of
aluminum, Environ. Health. Perspect. 65:363-441
Ghergariu S., Pop A., Kadar L., Sînu Marina – Manual de laborator
clinic veterinar, Ed.ALL Aducational, Bucuresti, 2000.
Graf, H., Stummvol, H.K., Meisinger, V. (1981)- Desferrioxamineinduced changes of aluminum kinetics during hemodialysis, Eur. Dial. Transplant
Assoc. 18:674-680
Graf, H., Stummvol, H.K., Meisinger, V. (1982)- Dialysate
aluminum concentration and aluminum transfer during hemodialysis., Lancet 1:46-
Jouhanneau, P., Raisbeck, G.M., Yiou, F., Lacour, B. et al. (1997)-
Gastrointestinal absorption, tissue retention, and urinary excretion of dietary
aluminum in rats determined by using 26Al, Clin. Chem. 43:1023-1028
Kaehny, W.D. et al. (1977b)- Aluminum transfer during hemodialysis,
Kidney Int. 12:361-365
Lione, A. (1983)- The prophylactic reduction of aluminum intake,
Food Chem. Toxicol. 21:103-109
Martin, R.B. (1986)- The chemistry of aluminum as related to biology
and medicine, Clin. Chem. 32:1797-1806
Minami, T., Ichii, M., Tohno, Y., Tohno, S., et al. (1996)- Agedependent aluminum accumulation in the human aorta and cerebral artery. Biol.
Trace Elem. Res. 55:199-205
Murakani, K., Yoshino, M. (2004)- Aluminum decreases the
glutathione regeneration by the inhibition of NADP-isocitrate dehydrogenase in
mitochondria, J.of Cellular Biochemistry, vol.93, Issue 6, 1267-1271
Pennington, J.A.T. (1987)- Aluminum content of foods and diets, Food
Add. Contamin. 5:161-232
Reller, H.J. and Luedders, W.L. (1977)- Dermatology and
Pharmacology, Adv. Med. Toxicol., Vol. 4, Marralli and Miaback Hemisphere
Publ. Corp., Washington, D.C.
Rozas, U.V., Port, F.K. and Easterling, R.E. (1978)- An
outbreak of dialysis dementia due to aluminum in the dialysate, J. Dialysis
:549-470
Sorenson, J.R., Campbell, J.R, Tepper, L.B. and Lingg, R.D.
(1974)- Aluminum in the environment and human health, Environ. Health Perspect.
:3-95.
