THE COMPARISON BETWEEN FOUR POTATO CULTIVARS MULTIPLE AXILLARY BUD MICROPROPAGATION SYSTEM EFFICIENCY
Keywords:
micropropagation, axillary bud, hormonal balance, culture mediumAbstract
The growth in vitro of several potato cultivars (Amelia, Christian, Nicoleta and
Roclas) on media containing plant growth regulators has been studied with a view to
accelerating micropropagation of slow-growing cultivars. Whilst 0,5 mg/l GA3+0.1
mg/l IAA substantially increased the height of plantlets of most cultivars, the
combination of 0,1 mg/l GA3+0,1 mg/l IAA+ 1,5 mg/l BAP was more effective in
increasing the number of shoots which could subsequently be cultured. Using PM
medium, a specific medium designed for potato, slow-growing or recalcitrant
cultivars, such as Amelia, could be multiplied more rapidly than on basic Murashige
& Skoog medium. Cultivars studied showed wide variation in their response to the
plant regulators, best results being obtained for the cultivar Nicoleta.
References
Bajaj, Y.P.S. (1992)- Biotechnology of nutritional improvement in potato, In:
Biotechnology in Agriculture and Forestry 3, 136-150.
Bhojwani., S.S., Razdan., M.K. (1996) - Plant Tissue Culture: Theory and
Practice. Elsevier, The Netherlands.
CachiŃă-Cosma D., Deliu, C., Rakosy-Tican, L., Ardelean A. (2004) - Tratat
de biotehnologie vegetală, Editura Dacia, Cluj-Napoca.
Danci, M. and Danci, O. (2007) - Ghid practic de culturi in vitro, Ed. Eurobit,
Timisoara.
Del Avila, A., Pereyra, S. M. and Argüello, J. A. (1996) - Potato
micropropagation: Growth of cultivars in solid and liquid media, Potato
Research 39: 253-258.
Dumont J. (1993) - Etude et perspectives d’utilisation de la microtuberation
in vitro en masse de la pomme de terre (Solanum tuberosum L.), Sciences
Agronomiques, Gembloux, 25-39.
Espinoza, N., Lizarraga, R., Siguenas, C., Buitron, F. (1992) - Tissue
culture: Micropropagation, Conservation and Export of Potato Germplasm. CIP
Research Guide 1, International Potato Center, Lima, Peru.
Holdgate, D.P. (1982) - Tissue culture for commercial plant propagation,
Span, 25 (1), 14-26.
Loebenstein, G., Alper, M. (1985) - Phytopathology, 53: 349 NeamŃu, G.,
Irimie, F. (1991). Fitoregulatori de creştere, Editura Ceres, Bucureşti
Ranalli, P. (1997). Innovative propagation methods in seed tuber
multiplication programmes. Potato Research 40: 439-453.