STUDIES REGARDING CULTURE MEDIUM INFLUENCE ON IN VITRO REGENERATION FROM WHEAT IMATURE EMBRYOS
Keywords:
immature embryos, culture medium, callus, regenerated plantsAbstract
Surnamed „embryos’ saving method”, embryos culture is an in vitro technique used
for over half of the century for saving the distant hybridization products, that would
have degenerate in other conditions. Immature embryos culture is used for initiation
of in vitro cultures imposed by the impossibility of using other explants for some of
the plant species. Wheat is one of the crops that immature embryos culture
technique is suitable for. This methods principle is based on aseptic embryos
excision and their inoculation to an adequate culture medium. In vitro culture
results depend in a greater manner of the basic culture medium and the hormonal
balance used. Immature embryos isolated from two Romanian wheat cultivars –
Dropia and Lovrin 41 – were inoculated for callus production on two types of basic
media added with 2,4 D. The selected calluses were transferred on MS basic
medium and several parameters were registered. Both cultivars emphasized a good
callusing capacity, in a different percentage depending on the culture media used,
such as 71,09 – 94,45%.. big differences between the cultivars regarding
embriogenic callus frequency, shooting callus frequency and regenerated plants
percentage were registered.
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