RESEARCH CONCERNING THE GENETIC STRUCTURE OF ROMANIAN SIMENTAL AND MARAMURES BROWN BREEEDS AT THE PITUITARY TRANSCRIPTION FACTOR LOCUS
Keywords:
polymorphism, gene, transcription factor, genetic structure, PCR, HinfIAbstract
Abstract: Pituitary transcription factor Pit-1, which belongs to a large POU domain
family is a positive regulatory factor of growth hormone, prolactin and thyrotropin
β-subunit in the mammalian pituitary. Therefore, the gene encoding Pit-1 was
chosen as a candidate gene to investigate its association with lactation performance
in cattle. The present study was carried out to establish the genetic structure at this
locus in two Romanian cattle breeds: Romanian Simmental and Maramures Brown,
to establish the possible association between genotype and milk yield and
conformation traits. A strategy employing polymerase chain reaction was used to
amplify a 1355- pb fragment from blood DNA and digestion with HinfI enzyme and
the genetic structure was estimated for both breeds.
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