THE PRIMER EXTENSION TECHNIQUE FOR THE POLYMORPHISM DETECTION AT OVINE PRN-P LOCUS
Keywords:
scrapie, prion, Prnp locus, primer extension, TSEAbstract
Scrapie is a prionic illness with endemic character in many parts of the glob, and the
control measures is difficult to apply because of the long incubation period, the lack
of the preclinical manifestation and the existing tests for diagnostic in living
animals. The Ppn-p locus is polymorphic with known variability at codon 136, 154,
171, which are associated with different sensibility in experimental and natural
spongiform encephalopaties. General the possible combinations of the 5 amino acids
encoded by the 3 different codons will determine the existence of 15 possible
genotypes. To put in evidence those polymorphisms at the ovine Prn-p locus, several
methods are developed but the most accurate assay is the direct sequencing of the
gene and the primer extension technique. The purpose of this study was to determine
the genotypes at Prp locus in 123 male of Tsurcana breed, Hateg ecotype, using
primer extension technique (ABI 3130xl Genetic Analyzer) and to establish the risk
groups of the susceptibility at scrapie disease.
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Protocol ABI Prism SnapShot TM Multiplex kit.
