OBSERVATIONS REGARDING OOCYTES STORAGE POST MENDING FROM SLAUGHTER FEMALES
Keywords:
pig, oocytes, viability, IVMAbstract
The oocytes viability must be taken as an important selection parameter for
successful in vitro cultivation. The ovaries were collected from the
slaughterhouse and maintained at 4°C for 7 days. Fallowing cumulus -
oocytes complexes recovery the viability was tested by two staining
methods. For the first experiment we used 27 cumulus - oocytes complexes,
stained with Neutral red and for the second experiment we used 11 cumulus
- oocytes complexes stained with Trypan blue. Fallowing staining with
Neutral red 23 cumulus - oocytes complexes were assessed as viable (were
stained in red – enzymatic activity within the cells) and for the Trypan blue
staining 11 cumulus - oocytes complexes were assessed as viable (remained
unstained – integers cellular membranes).
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