ELECTROPORATION OF CONFLUENT HM-1 ES CELLS LEADS TO HIGHER AMOUNT COLONIES

Abstract

Electroporation is used to induce homologous recombination in the genome of the
murine ES (embryonic stem) cells. Routinelly subconfluent ES cells are
recommended to be used in such experiments. Electroporation of immunoglobulin
specific targeting vectors with different length of homology leads to reduced number
of selected colonies. The enrichment of double selected colonies is high and thus the
amount of HM-1 ES cell colonies for the analysis is very low. Here we show that the
electroporation of confluent HM-1 ES cells leads to an increased amount of simple
and double selected colonies.