CHARACTERIZATION AT PROTEIN LEVEL OF THE NEW αS1 CASEIN ALLELE IRV DISCOVERED IN ROMANIAN GREY STEPPE CATTLE BREED MOLDAVIAN VARIETY
Keywords:
Romanian Grey Steppe Cattle, αS1-casein IRV, genetic polymorphism, cattle phylogenetic relationshipsAbstract
In this paper we describe the characterization at the protein level of αS1-casein I RV
allele discovered in Romanian Grey Steppe Cattle Moldavian variety, an autochthon
Bos Taurus Primigenius breed belonging Grey cattle family. In order to identify the
aminoacid/s substitution/s, responsible/s for the appearance of this new αS1 variant,
milk samples, collected from 2 cows previously identified as carriers of mutant αS1
allele in heterozygous condition (BIRV, CIRV) and 2 reference samples (BB,CC), were
purified by RP-HPLC. After purification, αS1-casein fraction collected from each
sample was submitted to hydrolysis and tryptic hydrolysates were analyzed by mass
spectrometry (MALDI-Tof and Orbitrap). The only possible substitution found is
concerning the glutamine (Q) residue codified by codons from exons 8, 9 or 11. This
is under study also at DNA level. This discovery could clarify the phylogenetic
relationships between Romanian Grey Steppe Cattle, Moldavian Variety (autochthon
Bos Taurus Primigenius) and their cousins from Africa and Asia.
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