Diploid Gynogenesis Induced by Heat Shock after Activation with UV-Irradiated Sperm in Rainbow Trout (Oncorhynchus Mykiss)

Authors

  • Ioan Bencsik Faculty of Animal Sciences and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine, 300645, Timişoara, Calea Aradului 119, Romania
  • Nicolae Pacala Faculty of Animal Sciences and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine, 300645, Timişoara, Calea Aradului 119, Romania
  • Rodica Caprita Faculty of Animal Sciences and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine, 300645, Timişoara, Calea Aradului 119, Romania
  • Dorel Dronca Faculty of Animal Sciences and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine, 300645, Timişoara, Calea Aradului 119, Romania
  • Gabi Dumitrescu Faculty of Animal Sciences and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine, 300645, Timişoara, Calea Aradului 119, Romania
  • Alena A. Bencsik-Theilen 2Institute of Medical Radiation Biology, University of Duisburg-Essen Medical School, 45122, Essen, Hufelandstr
  • Valeriu Caraba Faculty of Animal Sciences and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine, 300645, Timişoara, Calea Aradului 119, Romania
  • Liliana Petculescu-Ciochina Faculty of Animal Sciences and Biotechnologies, University of Agricultural Sciences and Veterinary Medicine, 300645, Timişoara, Calea Aradului 119, Romania

Keywords:

gynogenesis, dipoidy, triploidy, rainbow trout, heat shock

Abstract

Gynogenesis is a technique used to generate diploid individuals with genetic material exclusively of maternal origin. In this study we test the hypothesis that heat shock treatment of rainbow trout eggs, activated with UV irradiated sperms, leads to the generation of a uniform populations of diploid gynogenetic females. Activation of fertilized eggs with UV irradiated semen, by heat shock at 27.5°C for 10 min. directly after fertilization induces diploid gynogenesis. Diploid individuals are obtained at a rate of 73.33%. Triploid individuals are obtained at a rate of 26.67%. The enhanced number of triploid individuals may be due to insufficient irradiation of heritable material of sperm.

References

Bohl, M., Zucht und Production von Suβwasserfischen, Verglas Union Agrar,1999, p. 555-558.

Jian-He Xu, Feng You, Bin-Lun Yan, Pei-Jun Zhang, Effect of ultra-violet irradiation on sperm motility and diploid gynogenesis induction in large yellou croaker (Pseudosciaena crocea) undergoing cold shock , Aquacult.Int., Springer Sciences, 2007,DOI 10.1007/s10499-007-9101-6

Quillet, E., Aubard, G., Queau, I., Mutattion in a sex-determining gene in rainbow trout Detection and genetic analysis, 2002, The American Genetic Association 93:91–99

Colihueque, N., Iturra, P., Diaz, N., Veloso, A., Estay, F., Karyological analysis and identification of heterochromosomes in experimental gynogenetic offspring of rainbow trout (Oncorhynchus mykiss, Walbaum) 1992, Rev.Brasil.Genet.15,3,535-546.

Dunham, R.A., Aquaculture and fisheries biotehnologie, Genetic approaches, 2004, CABI Publishing, Cambridge, USA. ISBN 0 85199-596-9

Chourraut, D., Gynogenesis caused by ultraviolet irradiation of salmonid sperm, The Journal of experimental zoology , 1982, 223:175-181.

Kapuscinski A., R., Miller L., M., Genetic guidelines for fisheries management, 2007, University of Minesota, p.66-68.

Feist, G., Scheck, C. B., Chrrett, A., Controlling the sex of salmonids, 1996, Oregon Sea Grant, ISBN 1-881826-10-4.

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Published

2023-11-01

Issue

Vol. 44 No. 1 (2011): Scientific Papers: Animal Science and Biotechnologies

Section

Animal Reproduction

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.